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Rat Pancreatic prohormone(PPY) ELISA kit

文獻引用 (1) 說明書下載

產品詳情

產品描述：

大鼠胰多肽酶聯免疫試劑盒（CSB-E12747r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿樣本中的PPY含量。PPY即多肽YY，是由腸道L細胞分泌的一種激素。它能調節食欲、能量代謝等。研究機制上，其可與特定受體結合，作用于下丘腦等腦區以抑制食欲；也能影響胃腸道運動和消化液分泌，在維持機體能量穩態等生理過程中發揮重要作用。試劑盒檢測范圍為0.78 pg/mL-50 pg/mL，適用于科研場景中探究胰多肽在消化系統疾病模型、代謝調控機制或藥物干預實驗中的動態變化，為動物實驗提供可靠的內分泌指標檢測方案。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

PPY
Uniprot No.：

P06303
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma
檢測范圍：

0.78 pg/mL-50 pg/mL
靈敏度：

0.2 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat PPY in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	104
	Range %	93-109
1:200	Average %	100
	Range %	91-104
1:400	Average %	97
	Range %	88-101
1:800	Average %	90
	Range %	82-94

回收率：

The recovery of rat PPY spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	83-94
EDTA plasma (n=4)	100	91-104

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Pancreatic hormone is synthesized in pancreatic islets of Langerhans and acts as a regulator of pancreatic and gastrointestinal functions.
亞細胞定位：

Secreted.
蛋白家族：

NPY family
數據庫鏈接：

KEGG: rno:24677

STRING: 10116.ENSRNOP00000028318

UniGene: Rn.9755