Rat Neuron-specific enolase,NSE ELISA Kit

Rat Neuron-specific enolase,NSE ELISA Kit

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中文名稱：

大鼠神經特異性烯醇化酶(NSE)酶聯免疫試劑盒
貨號：

CSB-E07963r
規格：

96T/48T
價格：

￥3900/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產品描述：

大鼠神經特異性烯醇化酶(NSE)酶聯免疫試劑盒（CSB-E07963r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的ENO2含量。ENO2即烯醇化酶 2，在神經組織等中高表達。它參與糖酵解過程，為細胞提供能量。異常表達與神經母細胞瘤等腫瘤發生發展相關，可作為腫瘤診斷標志物與治療靶點，研究其機制有助于開發針對性治療策略。試劑盒檢測范圍為0.625 ng/mL-40 ng/mL，適用于實驗動物模型（如腦損傷、神經退行性疾病）中NSE的動態監測，或體外培養神經細胞的功能研究，可支持多種樣本類型的批量檢測，為探索神經系統疾病機制、評估神經損傷程度或藥物干預效果提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Eno2 ELISA Kit; Eno-2 ELISA Kit; Gamma-enolase ELISA Kit; EC 4.2.1.11 ELISA Kit; 2-phospho-D-glycerate hydro-lyase ELISA Kit; Enolase 2 ELISA Kit; Neural enolase ELISA Kit; Neuron-specific enolase ELISA Kit; NSE ELISA Kit
縮寫：

ENO2
Uniprot No.：

P07323
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.625 ng/mL-40 ng/mL
靈敏度：

0.156 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Metabolism
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat NSE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	89
	Range %	85-94
1:2	Average %	100
	Range %	97-104
1:4	Average %	90
	Range %	87-94
1:8	Average %	100
	Range %	95-103

回收率：

The recovery of rat NSE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	91-98
EDTA plasma (n=4)	95	92-97

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
40	2.260	2.232	2.246	2.078
20	1.454	1.486	1.470	1.302
10	0.920	0.928	0.924	0.756
5	0.654	0.663	0.659	0.491
2.5	0.403	0.398	0.401	0.233
1.25	0.322	0.314	0.318	0.150
0.625	0.266	0.257	0.262	0.094
0	0.170	0.166	0.168

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

Glycyrrhiza uralensis Fisch. and its active components mitigate Semen Strychni-induced neurotoxicity through regulating high mobility group box 1 (HMGB1) translocation X Duan,Biomedicine & pharmacotherapy,2023
Reversing Postcardiopulmonary Bypass Associated Cognitive Dysfunction Using k-Opioid Receptor Agonists to Regulate Microglial Polarization via the NLRP3/Caspase-1 Pathway Pei Song,Journal of Healthcare Engineering,2021
Dexmedetomidine reduces the neuronal apoptosis related to cardiopulmonary bypass by inhibiting activation of the JAK2?STAT3 pathway Chen Y.et al,Drug Des Devel Ther,2017
Magnetic resonance spectroscopy for assessment of brain injury in the rat model of sepsis Miaoyun Wen.et al,Experimental and Therapeutic Medicine,2017
Hypothermia followed by rapid rewarming exacerbates ischemia-induced brain injury and augments inflammatory response in rats. Zhu SZ.et al,Biochem Biophys Res Commun.,2016
Endotoxin-induced lung alveolar cell injury causes brain cell damage Rodr鉚guez-Gonz謾lez R.et al,Exp Biol Med (Maywood).,2015
Understanding the anti-kindling role and its mechanism of Resveratrol in Pentylenetetrazole induced-kindling in a rat model Saha L et al,Pharmacol Biochem Behav.,2014
Therapeutic time window of hypothermia is broader than cerebral artery flushing in carotid saline infusion after transient focal ischemic stroke in rats /,Neurological Research,,2012
Interrupted intracarotid artery cold saline infusion as an alternative method for neuroprotection after ischemic stroke Ya-Bin Ji et al,Neurosurgical Focus,2012

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功能：

Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival.
亞細胞定位：

Cytoplasm. Cell membrane.
蛋白家族：

Enolase family
組織特異性：

The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.
數據庫鏈接：

KEGG: rno:100911625

STRING: 10116.ENSRNOP00000005601

UniGene: Rn.10828

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.