Rat N-terminal procollagen Ⅲ propeptide,PⅢNP ELISA Kit

Rat N-terminal procollagen Ⅲ propeptide,PⅢNP ELISA Kit

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中文名稱：

大鼠Ⅲ型前膠原氨基末端肽(PⅢNP)酶聯免疫試劑盒
貨號：

CSB-E08096r
規格：

96T/48T
價格：

￥3900/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產品描述：

大鼠Ⅲ型前膠原氨基末端肽(PⅢNP)酶聯免疫試劑盒（CSB-E08096r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的PⅢNP含量。PⅢNP（Ⅲ型前膠原氨基端肽）是一種與組織纖維化相關的生物標志物。它在多種疾病如慢性心力衰竭和腦積水的發展過程中扮演重要角色。研究顯示，PⅢNP水平與疾病嚴重程度相關，可作為評估預后的指標。其具體作用機制涉及細胞外基質重塑和纖維化過程。試劑盒檢測范圍為0.625 ng/mL-40 ng/mL，適用于科研領域對膠原代謝相關機制的研究，適用于動物模型中的纖維化疾病機制探索、抗纖維化藥物研發效果評估，以及組織工程研究中膠原代謝動態監測。通過檢測不同樣本類型中的PⅢNP水平，研究者可深入探究膠原沉積與降解平衡在疾病發生發展中的作用，為相關科研項目提供可靠數據支持。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

N-terminal procollagen 3 propeptide,P3NP,_x000D_ N-terminal procollagen III propeptide,PIIINP
縮寫：

PⅢNP
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.625 ng/mL-40 ng/mL
靈敏度：

0.156 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Others
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat PⅢNP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	97
	Range %	92-101
1:2	Average %	98
	Range %	93-102
1:4	Average %	92
	Range %	87-95
1:8	Average %	95
	Range %	91-98

回收率：

The recovery of rat PⅢNP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	85-94
EDTA plasma (n=4)	98	94-105

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
40	2.102	2.077	2.090	1.959
20	1.412	1.447	1.430	1.299
10	0.924	0.945	0.935	0.804
5	0.592	0.608	0.600	0.469
2.5	0.360	0.374	0.367	0.236
1.25	0.289	0.271	0.280	0.149
0.625	0.211	0.222	0.217	0.086
0	0.130	0.132	0.131

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

MicroRNA-29a suppresses cardiac fibroblasts proliferation via targeting VEGF-A/MAPK signal pathway. Tao H.et al,Int J Biol Macromol.,2016

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.