Rat Low Density Lipoprotein cholesterol (LDL-C) ELISA kit

Rat Low Density Lipoprotein cholesterol (LDL-C) ELISA kit

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文獻引用 (9) 說明書下載

中文名稱：

大鼠低密度脂蛋白膽固醇(LDL-C)酶聯免疫試劑盒
貨號：

CSB-E16561r
規(guī)格：

96T/48T
價格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產品描述：

大鼠低密度脂蛋白膽固醇(LDL-C)酶聯免疫試劑盒（CSB-E16561r）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的LDL-C含量。LDL-C（低密度脂蛋白膽固醇）作為降脂治療的重要靶點，其研究背景源于其對心血管疾病風險的預測作用。研究機制主要圍繞LDL-C在動脈粥樣硬化形成中的作用，通過降低LDL-C水平來預防心血管疾病。試劑盒檢測范圍為125 ng/mL-8000 ng/mL，適用于實驗室開展動脈粥樣硬化模型評估、脂質代謝通路研究、降脂藥物篩選等科研項目，為心血管疾病基礎研究及營養(yǎng)學實驗提供可靠數據支持。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

LDL-C
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

125 ng/mL-8000 ng/mL
靈敏度：

62.5 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cardiovascular
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat LDL-C in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	98
	Range %	94-102
1:200	Average %	94
	Range %	91-97
1:400	Average %	102
	Range %	98-106
1:800	Average %	88
	Range %	84-92

回收率：

The recovery of rat LDL-C spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	89-99
EDTA plasma (n=4)	103	98-109

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average
8000	0.089	0.088	0.089
4000	0.182	0.178	0.180
2000	0.376	0.369	0.373
1000	0.630	0.642	0.636
500	1.130	1.146	1.138
250	1.528	1.516	1.522
125	1.914	1.810	1.862
0	2.267	2.169	2.218

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

Synergistic effects of pro-biotics or post-biotics with n-3 highly unsaturated fatty acids on chronic mild stress-induced dyslipidemia in rats J Lee, Y Jin, Y Park,Research Square,2024
The monomer of resveratrol butyrate ester ameliorates obesity and gut microbiota in high-fat diet rats YW Chen,Journal of Functional Foods,2024
Body composition and metabolic consequences of antibiotics most frequently administered to newborns in intensive care units: an experimental study in healthy newborn rats E Segura,Frontiers in medicine,2024
Ethanolic Extract of Orange Leaves Ameliorates Dyslipidemia in Streptozotocin-induced Diabetic Rats. N Dodantenna,/,2024
Body Composition and Metabolic Consequences of Most Frequent Antibiotics Given to Newborns in Intensive Care Units, an Experimental Study in Newborn Rats C Wang,/,2024
Alleviation of Limosilactobacillus reuteri in polycystic ovary syndrome protects against circadian dysrhythmia-induced dyslipidemia via capric acid and GALR1 signaling S Li,NPJ biofilms and microbiomes,2023
Eicosapentaenoic acid and docosahexaenoic acid, but not α-linolenic acid, decreased LDL-cholesterol synergistically with estrogen via regulation of cholesterol synthesis and clearance in ovariectomized rats ChungPark, et al,Nutrition research,2019
Palm Wine and Orijin Bitters Severed Hyperlipidemia and Immunomodulatory Responses in Atherogenic-diet Fed Male Wistar Rats Bukoye, Oyewo Emmanuel.et al,American Journal of Food Science and Health,2017
Effect of n-3 PUFA supplementation at different EPA: DHA ratios on the spontaneously hypertensive obese rat model of the metabolic syndrome Molinar-Toribio E et al,Br J Nutr,2015

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.