Rat Interleukin 9,IL-9 ELISA KIT

產(chǎn)品詳情

文獻(xiàn)引用 (2) 說(shuō)明書(shū)下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

大鼠白介素9(IL-9)酶聯(lián)免疫試劑盒（CSB-E07294r）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的IL-9含量。IL-9是重要的細(xì)胞因子，在免疫調(diào)節(jié)等方面發(fā)揮作用。其背景上，與多種疾病如哮喘、自身免疫病相關(guān)。研究機(jī)制方面，它通過(guò)與受體結(jié)合激活信號(hào)通路，影響細(xì)胞增殖、分化和功能，為相關(guān)疾病治療提供潛在靶點(diǎn)。試劑盒檢測(cè)范圍為15.6 pg/mL-1000 pg/mL，炎癥模型研究、免疫調(diào)控機(jī)制探索、藥物干預(yù)效果評(píng)價(jià)、研究IL - 9在呼吸系統(tǒng)疾病、寄生蟲(chóng)感染或自身免疫疾病中的功能本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
縮寫(xiě)：

IL-9
Uniprot No.：

D4A8I9
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates
檢測(cè)范圍：

15.6 pg/mL-1000 pg/mL
靈敏度：

3.9 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Immunology
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	97
	Range %	92-103
1:2	Average %	92
	Range %	88-96
1:4	Average %	95
	Range %	90-99
1:8	Average %	84
	Range %	80-88

回收率：

The recovery of rat IL-9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	85-95
EDTA plasma (n=4)	98	93-104

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.