Rat Interleukin 33(IL-33) ELISA Kit

Rat Interleukin 33(IL-33) ELISA Kit

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中文名稱：

大鼠白介素33(IL-33)酶聯免疫試劑盒
貨號：

CSB-E14077r
規格：

96T/48T
價格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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大鼠白介素33(IL-33)酶聯免疫試劑盒（CSB-E14077r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的IL33含量。IL-33是一種前炎癥細胞因子，屬于IL-1家族，主要在細胞損傷時釋放，誘導Th2型炎癥反應。其與ST2受體結合后，激活下游信號通路，調節多種免疫細胞功能。IL-33在腫瘤、自身免疫疾病和過敏性疾病中發揮重要作用。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL，適用于基礎科研中炎癥機制研究、免疫調控模型分析以及藥物干預效果評估等方向，例如在呼吸道炎癥模型、自身免疫疾病或創傷修復實驗中定量分析IL - 33的動態變化。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Il33Interleukin-33 ELISA kit; IL-33 ELISA kit
縮寫：

IL33
Uniprot No.：

Q66H70
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

15.6 pg/mL-1000 pg/mL
靈敏度：

3.9 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-33 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	103
	Range %	98-108
1:2	Average %	96
	Range %	91-101
1:4	Average %	89
	Range %	83-95
1:8	Average %	98
	Range %	93-104

回收率：

The recovery of rat IL-33 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	85-94
EDTA plasma (n=4)	94	90-99

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	2.981	2.998	2.990	2.842
500	1.967	1.873	1.920	1.772
250	1.333	1.427	1.380	1.232
125	0.845	0.835	0.840	0.692
62.5	0.565	0.559	0.562	0.414
31.2	0.331	0.348	0.340	0.192
15.6	0.225	0.236	0.231	0.083
0	0.143	0.153	0.148

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

Dihydromyricetin treats pulmonary hypertension by modulating CKLF1/CCR5 axis-induced pulmonary vascular cell pyroptosis Q Yan, P Li, S Liu, Y Sun, C Chen, J Long, Y Lin,Biomedicine & Pharmacotherapy,2024
Effect of Src tyrosine kinase on a rat model of asthma M Wu,Exp Ther Med,2022
Lycopene Modulates Placental Health and Fetal Development Under High‐Fat Diet During Pregnancy of Rats S Sun,Molecular Nutrition & Food Research,2021
The possible role of interleukin-33 as a new player in the pathogenesis of contrast-induced nephropathy in diabetic rats Demirtas L.et al,Ren Fail.,2016
Melatonin Attenuates Contrast-Induced Nephropathy in Diabetic Rats: The Role of Interleukin-33 and Oxidative Stress Didem Onk. et al,Mediators of Inflammation,2016

功能：

Cytokine that binds to and signals through the IL1RL1/ST2 receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells. Involved in the maturation of Th2 cells inducing the secretion of T-helper type 2-associated cytokines. Also involved in activation of mast cells, basophils, eosinophils and natural killer cells. Acts as a chemoattractant for Th2 cells, and may function as an 'alarmin', that amplifies immune responses during tissue injury.; In quiescent endothelia the uncleaved form is constitutively and abundantly expressed, and acts as a chromatin-associated nuclear factor with transcriptional repressor properties, it may sequester nuclear NF-kappaB/RELA, lowering expression of its targets. This form is rapidely lost upon angiogenic or proinflammatory activation.
基因功能參考文獻：
1. The release of IL-33 from necrotic cells might induce autophagy, which can further balance the effects of increased apoptosis secondary to contrast-induced nephropathy in diabetic kidney disease. PMID: 27055881
2. TNF-alpha stimulated IL-33/sST2 and subsequent increase of IL-6 may be the mechanism of IL-33 in acute pancreatitis. PMID: 26927343
3. poly-IC and IL-33 play a role in myelin repair by enhancing expression of myelin genes. PMID: 27022724
4. IL-33 protects against myocardial I/R injury by inhibiting inflammatory responses and myocardial apoptosis. PMID: 26571038
5. These findings indicate that IL-33 might play an important role in the inflammatory response following subarachnoid hemorrhage. PMID: 25417195
6. MRAs play a pivotal role in the Gal-3 and IL-33/ST2 modulation in post-MI cardiac remodeling. PMID: 25458175
7. Induced interleukin-33 expression enhances the tumorigenic activity of rat glioma cells. PMID: 24327583
8. Data indicate IL-33 concentration was increased in the pancreas in duct ligation-induced acute pancreatitis. PMID: 23418608
9. The effects of external application of IL1beta and IL33 on the cardiomyocyte transcriptome, were compared. PMID: 23219998
10. This study examined the expression of IL-33 and the IL-33-mediated regulation of cell functions in pancreatic stellate cells isolated from human and rat pancreas tissues. PMID: 20689058
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亞細胞定位：

Nucleus. Chromosome. Cytoplasm. Cytoplasmic vesicle, secretory vesicle. Secreted.
蛋白家族：

IL-1 family
數據庫鏈接：

KEGG: rno:361749

UniGene: Rn.106849

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.