Rat Interleukin 23,IL-23 ELISA Kit

Rat Interleukin 23,IL-23 ELISA Kit

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文獻引用 (11) 說明書下載

中文名稱：

大鼠白介素23(IL-23)酶聯免疫試劑盒
貨號：

CSB-E08462r
規格：

96T/48T
價格：

￥3200/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產品描述：

大鼠白介素23(IL-23)酶聯免疫試劑盒（CSB-E08462r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養上清液、組織勻漿樣本中的IL-23含量。IL-23是一種細胞因子，在免疫系統中發揮重要作用。其與炎癥、自身免疫性疾病密切相關，如銀屑病、炎癥性腸病等。研究機制顯示，它可激活特定的T細胞亞群，促進炎癥因子的產生，引發免疫反應，目前是藥物研發的熱門靶點。試劑盒檢測范圍為3.12 pg/mL-200 pg/mL，適用于體外炎癥模型研究、免疫通路調控實驗或藥物干預效果評估等科研領域，為探索IL - 23在神經免疫、腸道屏障功能或慢性炎癥反應中的分子機制提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

N/A
縮寫：

IL-23
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates
檢測范圍：

3.12 pg/mL-200 pg/mL
靈敏度：

0.78 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-23 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	89-94
1:2	Average %	104
	Range %	100-107
1:4	Average %	92
	Range %	87-95

回收率：

The recovery of rat IL-23 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	89-95
EDTA plasma (n=4)	98	94-100

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
200	2.147	2.112	2.130	1.998
100	1.851	1.776	1.814	1.682
50	1.308	1.288	1.298	1.166
25	0.903	0.898	0.901	0.769
12.5	0.621	0.614	0.618	0.486
6.25	0.375	0.359	0.367	0.235
3.12	0.236	0.224	0.230	0.098
0	0.134	0.130	0.132

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

Regulation of Dendritic Cell Function by Ermiaosan via the EP4-cAMP-CREB Signaling Pathway JM Ding, L Min, W Jin, SM Cheng, XW Meng,arXiv,2025
Imatinib mitigates experimentally-induced ulcerative colitis: Possible contribution of NF-kB/JAK2/STAT3/COX2 signaling pathway M Shalaby,Life sciences,2023
JAK2 inhibitor ameliorates the progression of experimental autoimmune myasthenia gravis and balances Th17/Treg cells via regulating the JAK2/ STAT3-AKT/mTOR signaling pathway Y Lu,International immunopharmacology,2023
Egg white hydrolysate from simulated gastrointestinal digestion alleviates the inflammation and improves the nutritional status in TNBS-induced Crohn’s disease rats T Wu,Journal of Functional Foods,2022
Mesenchymal stem cells moderate experimental autoimmune uveitis by dynamic regulating Th17 and Breg cells response Yajun Mu,Journal of Tissue Engineering and Regenerative Medicine,2021
The Protective Effects of the Ethyl Acetate Part of Er MiaoSan on Adjuvant Arthritis Rats by Regulating the Function of Bone Marrow-Derived Dendritic Cells J Ding,Evidence-Based Complementary and Alternative Medicine,2020
Kuijieling regulates the differentiation of Treg and Th17 cells to ameliorate experimental colitis in rats YuLong.et al,Biomedicine & Pharmacotherapy,2018
Synthetic p55 tandem DNA vaccine against Pneumocystis carinii in rats. Fan H.et al,Microbiol Immunol.,2016
Matrine improves cognitive impairment and modulates the balance of Th17/Treg cytokines in a rat model of A尾1-42-induced Alzheimer's disease Zhang Y.et al,Cent Eur J Immunol.,2016
Mast cells mediate Pseudomonas aeruginosa lipopolysaccharide-induced lung inflammation in rat B. V. Lê,Tumor Biology,2012
Modulation of Pseudomonas aeruginosa lipopolysaccharide-induced lung inflammation by chronic iron overload in rat Bá Vuong Lê,International Journal of Rheumatic Diseases,2012

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■ 常見問題解答

I am interested in measuring cytokines in patients affected by autoimmune thyroid diseases. For instance, i found the kit
(Human Interleukin 23 (IL-23) ELISA Kit,Catalog Number. CSB-E08461h) of your company,
Can this kit be used for the same purpose in lab animals (mouse, rats), if so, we are also interested in measuring IL-21, IL-22, IL-17A, IL-17F, all relevant kits produced by the same company, can they also be used for the same intention in lab animals??

Thanks for your inquiry. For these cytokines IL-23, IL-21, IL-22, IL-17A, IL-17F,detection of cross-species is not advised,as it will be affected by the characteristics of antibodies and the matrix difference.
Regarding your research in measuring cytokines in patients affected by autoimmune thyroid diseases,we suggest you use human kits.While regarding your early laboratory research on lab animals,we suggest you use the corresponding lab animals kits.
Human kits:
#CSB-E08461h,Human Interleukin 23,IL-23 ELISA Kit
#CSB-E11707h,Human Interleukin 21,IL-21 ELISA Kit
#CSB-E13418h,Human Interleukin 22(IL-22) ELISA KIT
#CSB-E12819hHuman Interleukin 17A (IL-17A/IL-17) ELISA Kit
#CSB-E15916h,Human Interleukin17F (IL17F) ELISA Kit
Mouse kits:
#CSB-E08463m,Mouse Interleukin 23,IL-23 ELISA Kit
#CSB-E13162m,Mouse Interleukin 21(IL-21) ELISA Kit
#CSB-E13513m,Mouse Interleukin 22(IL-22) ELISA Kit
#CSB-E04608m,Mouse Interleukin 17,IL-17 ELISA Kit
#CSB-EL011601MO,Mouse Interleukin-17F(IL17F) ELISA kit
Rat kits:
#CSB-E08462r,Rat Interleukin 23,IL-23 ELISA Kit
#CSB-E11843r,Rat Interleukin 21,IL-21 ELISA Kit
#CSB-E14918r,Rat Interleukin 22(IL-22) ELISA Kit
#CSB-E07451r,Rat Interleukin 17,IL-17 ELISA KIT
#CSB-EL011601RA,Rat Interleukin-17F(IL17F) ELISA kit

If you are interested to order any one of them,we will foward your request to local distributor.Well,could you advise which coutry are you from?Thank you very much!

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.