Rat Interleukin 21,IL-21 ELISA Kit

產(chǎn)品詳情

文獻引用 (5) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

大鼠白介素21(IL-21)酶聯(lián)免疫試劑盒（CSB-E11843r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的IL21含量。IL21，即白細(xì)胞介素-21，是一種由CD4+T細(xì)胞和自然殺傷T(NKT)細(xì)胞產(chǎn)生的細(xì)胞因子，屬于IL-15/IL-21家族。它通過結(jié)合IL-21R受體激活JAK1和JAK3信號，進而調(diào)節(jié)T、B、NK細(xì)胞和DC的增殖與分化，在免疫調(diào)節(jié)中發(fā)揮重要作用。IL21在多種免疫疾病中扮演關(guān)鍵角色，如自身免疫性疾病和癌癥。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL，適用于體外定量分析大鼠外周血或組織樣本中IL-21的動態(tài)變化，可廣泛應(yīng)用于免疫調(diào)節(jié)機制研究、炎癥性疾病動物模型評估或藥物干預(yù)后免疫指標(biāo)變化的科研分析本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

Il21Interleukin-21 ELISA Kit; IL-21 ELISA Kit
縮寫：

IL21
Uniprot No.：

A3QPB9
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

15.6 pg/mL-1000 pg/mL
靈敏度：

3.9 pg/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-21 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	85-96
1:2	Average %	89
	Range %	84-95
1:4	Average %	103
	Range %	99-108
1:8	Average %	99
	Range %	94-105

回收率：

The recovery of rat IL-21 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	92-101
EDTA plasma (n=4)	92	88-100

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Cytokine with immunoregulatory activity. May promote the transition between innate and adaptive immunity. Induces the production of IgG(1) and IgG(3) in B-cells. Implicated in the generation and maintenance of T follicular helper (Tfh) cells and the formation of germinal-centers. Together with IL6, control the early generation of Tfh cells and are critical for an effective antibody response to acute viral infection. May play a role in proliferation and maturation of natural killer (NK) cells in synergy with IL15. May regulate proliferation of mature B- and T-cells in response to activating stimuli. In synergy with IL15 and IL18 stimulates interferon gamma production in T-cells and NK cells. During T-cell mediated immune response may inhibit dendritic cells (DC) activation and maturation.
亞細(xì)胞定位：

Secreted.
蛋白家族：

IL-15/IL-21 family
數(shù)據(jù)庫鏈接：

KEGG: rno:365769

STRING: 10116.ENSRNOP00000023348

UniGene: Rn.214949