Rat Interleukin 16,IL-16 ELISA KIT

產品詳情

文獻引用 (2) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

大鼠白介素16(IL-16)酶聯免疫試劑盒（CSB-E07452r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的IL16含量。IL16作為一個靶點，其背景上是具有免疫調節功能的細胞因子。在研究機制方面，它能與CD4受體結合，參與T淋巴細胞等的活化、增殖與遷移過程，在免疫相關疾病研究中受關注，為疾病治療策略開發提供潛在方向。試劑盒檢測范圍為31.25 pg/mL-2000 pg/mL，適用于大鼠炎癥機制研究、免疫相關疾病模型評估及藥物干預效果分析等科研領域；可滿足細胞因子網絡研究、免疫微環境分析等實驗需求，尤其適用于神經科學、免疫學及藥理學領域的體外樣本檢測。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

IL-16
Uniprot No.：

G5BEZ3
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

31.25 pg/mL-2000 pg/mL
靈敏度：

7.81 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-16 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	87-96
1:2	Average %	98
	Range %	94-102
1:4	Average %	103
	Range %	98-107
1:8	Average %	89
	Range %	84-96

回收率：

The recovery of rat IL-16 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-98
EDTA plasma (n=4)	88	82-96

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.