Rat Insulin-like growth factor binding protein 1,IGFBP-1 ELISA Kit

Rat Insulin-like growth factor binding protein 1,IGFBP-1 ELISA Kit

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文獻引用 (1) 說明書下載

中文名稱：

大鼠胰島素樣生長因子結合蛋白1(IGFBP-1)酶聯(lián)免疫試劑盒
貨號：

CSB-E07349r
規(guī)格：

96T/48T
價格：

￥3200/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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靶點詳情

產(chǎn)品描述：

大鼠胰島素樣生長因子結合蛋白1(IGFBP-1)酶聯(lián)免疫試劑盒（CSB-E07349r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的IGFBP1含量。IGFBP1即胰島素樣生長因子結合蛋白1，它在調(diào)節(jié)細胞生長、分化和代謝等方面有重要作用。作為一種靶點，研究機制聚焦于其與胰島素樣生長因子的相互作用，以及對下游信號通路影響，以探尋其在腫瘤、代謝疾病等病理過程中的潛在干預方式。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL，適用于基礎科研領域，可用于研究IGFBP-1在大鼠模型中的表達調(diào)控機制，探索其在代謝性疾病、生長發(fā)育異常或組織損傷修復中的潛在作用，亦可用于體外藥物干預實驗或疾病模型構建中相關生物標志物的定量分析。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

Igfbp1 ELISA Kit; Igfbp-1 ELISA Kit; Insulin-like growth factor-binding protein 1 ELISA Kit; IBP-1 ELISA Kit; IGF-binding protein 1 ELISA Kit; IGFBP-1 ELISA Kit
縮寫：

IGFBP1
Uniprot No.：

P21743
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates
檢測范圍：

15.6 pg/mL-1000 pg/mL
靈敏度：

3.9 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat IGFBP-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	87
	Range %	80-94
1:200	Average %	97
	Range %	91-105
1:400	Average %	88
	Range %	81-95
1:800	Average %	100
	Range %	95-94

回收率：

The recovery of rat IGFBP-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-99
EDTA plasma (n=4)	92	87-97

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	2.142	2.287	2.215	2.030
500	1.882	1.897	1.890	1.705
250	1.297	1.324	1.311	1.126
125	0.803	0.815	0.809	0.624
62.5	0.492	0.506	0.499	0.314
31.2	0.360	0.387	0.374	0.189
15.6	0.273	0.288	0.281	0.096
0	0.184	0.186	0.185

數(shù)據(jù)處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

Levels of the IGF system components in malignant lesions of the lungs with preventive effect of 1, 3-diethylbenzimidazolium triiodide in the experiment LK Trepitaki,Journal of Clinical Oncology,2021

功能：

IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Promotes cell migration.
基因功能參考文獻：
1. In simulated sleep apnea hypopnea syndrome, intermittent hypoxia in young rats does not cause physical growth retardation, but serum IGF-1 and IGFBP-3 levels decreased with the increase of hypoxia and decline of oxygen saturation. PMID: 22805023
2. up-regulation of IGFBP-1 in oligodendrocytes in multiple sclerosis may serve two functions: (i) regulate IGF-1 actions, (ii) exert IGF-independent effects through its RGD sequence PMID: 20345750
3. Insulin regulation of insulin-like growth factor-binding protein-1 gene expression PMID: 11784721
4. regulation of expression by insulin is impaired by presence of hydrogen peroxide PMID: 11942857
5. IGFBP-1 and IGFBP-3 are present in phosphorylated form in fasted rat skin, which induced inhibition of collagen biosynthesis in cultured fibroblasts PMID: 12670795
6. IGFBP-1 released during the early steps of liver tissue damage and repair may interact with liver cells and potentiate the sensitivity of IGF-I to mitogenic signals. PMID: 15070850
7. In this report we demonstrate that in H4IIE-C3 cells, four distinct classes of GSK-3 inhibitor mimic the effect of insulin on a third thymine-rich insulin response element-containing gene, IGFBP-1. PMID: 15350195
8. Iron, radical oxygen species, and HIF-2 and -3 as well as prolyl hydroxylase pathways play important roles in mediating effects of hypoxia on IGFBP-1 gene expression in liver. PMID: 16166214
9. insulin inhibition of IGFBP-1 mRNA levels can occur in the absence of the phosphorylation of Foxo1/Foxo3, whereas activation of the mTOR pathway is both necessary and sufficient PMID: 16455781
10. Activation of the mTOR pathway, without activation of its upstream regulator PI 3-kinase, reduces IGFBP1 expression. PMID: 17032741
11. Age-related oxidative stress underlies the upregulated JNK activation and IGFBP-1 expression. PMID: 17645865
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亞細胞定位：

Secreted.
數(shù)據(jù)庫鏈接：

KEGG: rno:25685

STRING: 10116.ENSRNOP00000011338

UniGene: Rn.34026

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.