Rat Glypican-3(GPC3) ELISA kit

Rat Glypican-3(GPC3) ELISA kit

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中文名稱：

大鼠磷脂酰肌醇蛋白聚糖3(GPC3)酶聯免疫試劑盒
貨號：

CSB-EL009705RA
規格：

96T/48T
價格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產品描述：

大鼠磷脂酰肌醇蛋白聚糖3(GPC3)酶聯免疫試劑盒（CSB-EL009705RA）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的GPC3含量。GPC3是一種硫酸乙酰肝素糖蛋白，在正常成人肝臟中低表達，在肝癌等多種腫瘤組織中高表達，是極具潛力的腫瘤治療靶點。其研究機制主要圍繞開發針對GPC3的單克隆抗體、CAR-T細胞療法等，以實現對腫瘤細胞的精準殺傷。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL，適用于肝病模型研究、腫瘤微環境分析及靶向治療機制探索等科研場景，例如可通過監測藥物干預后GPC3表達水平變化評估治療效果，或結合組織樣本分析其在不同病理階段的生物學功能。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Gpc3 ELISA Kit; Glypican-3 ELISA Kit; Intestinal protein OCI-5) [Cleaved into: Glypican-3 alpha subunit; Glypican-3 beta subunit] ELISA Kit
縮寫：

GPC3
Uniprot No.：

P13265
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

15.6 pg/mL-1000 pg/mL
靈敏度：

3.9 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat GPC3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	85-99
1:2	Average %	107
	Range %	97-110
1:4	Average %	89
	Range %	83-93
1:8	Average %	99
	Range %	91-104

回收率：

The recovery of rat GPC3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	84-99
EDTA plasma (n=4)	100	94-106

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	2.506	2.415	2.461	2.280
500	2.091	1.974	2.033	1.852
250	1.353	1.332	1.343	1.162
125	0.813	0.798	0.806	0.625
62.5	0.484	0.456	0.470	0.289
31.2	0.408	0.392	0.400	0.219
15.6	0.274	0.267	0.271	0.090
0	0.183	0.179	0.181

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

功能：

Cell surface proteoglycan that bears heparan sulfate. Negatively regulates the hedgehog signaling pathway when attached via the GPI-anchor to the cell surface by competing with the hedgehog receptor PTC1 for binding to hedgehog proteins. Binding to the hedgehog protein SHH triggers internalization of the complex by endocytosis and its subsequent lysosomal degradation. Positively regulates the canonical Wnt signaling pathway by binding to the Wnt receptor Frizzled and stimulating the binding of the Frizzled receptor to Wnt ligands. Positively regulates the non-canonical Wnt signaling pathway. Binds to CD81 which decreases the availability of free CD81 for binding to the transcriptional repressor HHEX, resulting in nuclear translocation of HHEX and transcriptional repression. Inhibits the dipeptidyl peptidase activity of DPP4. Plays a role in limb patterning and skeletal development by controlling the cellular response to BMP4. Modulates the effects of growth factors BMP2, BMP7 and FGF7 on renal branching morphogenesis. Required for coronary vascular development. Plays a role in regulating cell movements during gastrulation.
基因功能參考文獻：
1. Abnormal oncofetal antigen GPC-3 and GPC-3 mRNA expression in hepatocarcinogenesis may be promising molecular markers for early diagnosis of hepatocellular carcinoma. PMID: 21669573
2. Gpc3 is a marker of hepatic progenitor cells and of early liver lesions. PMID: 17117158
3. GPC3 may be a negative regulator of liver regeneration and hepatocyte proliferation, and that this regulation may involve CD81. PMID: 19574424
亞細胞定位：

Cell membrane; Lipid-anchor, GPI-anchor; Extracellular side.
蛋白家族：

Glypican family
數據庫鏈接：

KEGG: rno:25236

STRING: 10116.ENSRNOP00000043344

UniGene: Rn.9717

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.