Rat Glutathione Peroxidase (GSH-PX) ELISA kit

Rat Glutathione Peroxidase (GSH-PX) ELISA kit

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文獻引用 (44) 說明書下載

中文名稱：

大鼠谷胱甘肽過氧化物酶(GSH-PX)酶聯免疫試劑盒
貨號：

CSB-E12146r
規格：

96T/48T
價格：

￥3800/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產品描述：

大鼠谷胱甘肽過氧化物酶(GSH-PX)酶聯免疫試劑盒（CSB-E12146r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的GPX1含量。GPX1是重要靶點。它是谷胱甘肽過氧化物酶家族成員，能催化還原型谷胱甘肽變成氧化型谷胱甘肽，清除體內過氧化物和自由基，保護細胞免受氧化損傷。在多種疾病尤其癌癥、神經退行性疾病研究中，其作用機制是研究熱點。試劑盒檢測范圍為12.5 mIU/mL-800 mIU/mL，適用于多種科研場景，包括但不限于評估氧化應激模型（如肝臟疾病、神經退行性病變等）中酶活性變化、藥物或環境因素對抗氧化系統的影響研究，以及代謝性疾病相關機制探索。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Gpx1Glutathione peroxidase 1 ELISA Kit; GPx-1 ELISA Kit; GSHPx-1 ELISA Kit; EC 1.11.1.9 ELISA Kit; Cellular glutathione peroxidase ELISA Kit
縮寫：

GPX1
Uniprot No.：

P04041
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

12.5 mIU/mL-800 mIU/mL
靈敏度：

3.12 mIU/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cancer
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat GSH-PX in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:200	Average %	92
	Range %	85-100
1:400	Average %	97
	Range %	94-101
1:800	Average %	95
	Range %	90-99
1:1600	Average %	93
	Range %	87-98

回收率：

The recovery of rat GSH-PX spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	86-100
EDTA plasma (n=4)	100	95-105

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

mIU/ml	OD1	OD2	Average	Corrected
800	2.251	2.299	2.275	2.119
400	1.845	1.834	1.840	1.684
200	1.301	1.324	1.313	1.157
100	0.892	0.876	0.884	0.728
50	0.553	0.590	0.572	0.416
25	0.395	0.381	0.388	0.232
12.5	0.251	0.234	0.243	0.087
0	0.155	0.157	0.156

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

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最新研究進展：

GPX1是一種谷胱甘肽過氧化物酶，可以清除細胞內的過氧化氫等活性氧，具有抗氧化和細胞保護作用。最新研究表明，GPX1在多種疾病的發生和發展中發揮重要作用。例如，一些研究發現GPX1與心血管疾病、癌癥和糖尿病等疾病的風險存在相關性。此外，一些研究還發現GPX1在免疫調節、神經保護和干細胞分化等方面發揮著重要作用。
功能：

Protects the hemoglobin in erythrocytes from oxidative breakdown. In platelets, plays a crucial role of glutathione peroxidase in the arachidonic acid metabolism.
基因功能參考文獻：
1. Knockdown of nNOS or GPx1 was specific and had broad effects on downstream genes, though it only modestly ameliorated traumatic brain injury-induced working memory deficits, despite widespread genomic changes. PMID: 29016640
2. The glutathione peroxidase activity and p110-PI3K/Thr308-Akt are both modulated in the same way by oxygen. PMID: 24401635
3. VPO1 plays an important role in senescence of endothelial cells and endothelial dysfunction by induction of oxidative stress and inflammatory reaction in type 2 diabetic rats. PMID: 26142204
4. glutathione peroxidase 1 immunoreactivity was diffusely cytoplasmic in some of the hepatocytes PMID: 24867493
5. Dietary Se-enriched A. bisporus protected against increase in ileum permeability and up-regulated GPx-1 and GPx-2 expression, selenoenzymes relevant to mitigating oxidative stress. PMID: 24962481
6. It represents the pivotal antioxidant enzyme in vascular endothelium and exerts a protective effect against the presence of coronary artery disease PMID: 24389817
7. data collectively indicate that GDNF is capable of inducing anti-oxidant activities of intra-cellular GPX-1 and that growth-promoting potential of GDNF and anti-oxidant properties of GPX-1 can, in concert, maximize survival of dopaminergic neurons. PMID: 23472740
8. Upon selenium fluctuation, the modulation of UGA recoding efficiency depends on the nature of the SECIS, with Gpx1 being more sensitive than Gpx4. PMID: 22614831
9. GPx levels in the cortex and hippocampus were increased in OLETF rats compared with LETO rats, with an inverse correlation between GPx in the hippocampus and learning score. PMID: 22981416
10. The glutathione peroxidase represents an important part of the antioxidant defense system. PMID: 20524817
11. expression of glutathione peroxidase 1 and glutathione reductase via GSH did not appear to coordinate with development, tissue localization or under oxidative stress PMID: 12516874
12. excess amounts of PLOOH are efficiently eliminated through the hydrolysis by PLA2, and the subsequent reduction of FA hydroperoxide by GPx is the critical step for complete detoxification of oxidized phospholipids in the stomach PMID: 12934674
13. Over-expression gpx1 genes in primary hippocampal or cortical cultures resulted in increased enzymatic activity of the cognate protein. PMID: 14713308
14. Gxp1 levels was increased indicating that a reduction in oxidative damage protection may be responsible for accelerated telomere shortening over time, resulting in increased cellular senescence, loss of renal function, and death in male rats. PMID: 16189290
15. Novel sites of Gpx1 expression were observed in colon lymphatic tissue. PMID: 16229841
16. Our results suggest that impairment of the GPx (including glutathione)-mediated antioxidant system contributed to epileptogenesis in GEPR-9s. PMID: 18226427
17. docosahexaenoic acid sensitizes breast cancer cells to anthracyclines through loss of glutathione peroxidase (GPx1) response PMID: 18267129
18. Selenium requirements are higher for glutathione peroxidase-1 mRNA than gpx1 activity in rat testis. PMID: 19234057
19. red blood cell glutathione peroxidase (Gpx1) activity in old female rats. PMID: 19398061
20. Whole blood selenoprotein mRNA levels can be used as molecular biomarkers for assessing Selenium requirements. PMID: 19855070
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亞細胞定位：

Cytoplasm.
蛋白家族：

Glutathione peroxidase family
組織特異性：

Expressed in liver and lung.
數據庫鏈接：

KEGG: rno:24404

STRING: 10116.ENSRNOP00000066577

UniGene: Rn.11323

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.