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中文名稱：大鼠胃泌素(Gastrin)酶聯免疫試劑盒
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貨號：CSB-E12743r
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規格：96T/48T
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價格：￥3600/￥2500
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其他：
產品詳情
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產品描述：大鼠胃泌素(Gastrin)酶聯免疫試劑盒（CSB-E12743r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養上清液,組織勻漿樣本中的GAST含量。GAST作為一種靶點，其背景涉及在生理過程中發揮重要作用，如調節胃腸道功能等。在研究機制方面，主要聚焦于其信號傳導通路，探索其與疾病發生、發展的關聯，旨在通過干預該靶點來開發針對相關疾病的治療策略和藥物。試劑盒檢測范圍為0.312 pg/mL-20 pg/mL，該產品適用于胃腸功能研究、藥物對胃泌素分泌影響的評價及消化系統疾病模型中激素水平動態分析本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
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別名：Gast ELISA Kit; Gas ELISA Kit; Gastrin [Cleaved into: Big gastrin ELISA Kit; Gastrin-34 ELISA Kit; G34); Gastrin] ELISA Kit
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縮寫：GAST
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Uniprot No.：
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種屬：Rattus norvegicus (Rat)
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樣本類型：serum, plasma, cell culture supernates ,tissue homogenates
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檢測范圍：0.312 pg/mL-20 pg/mL
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靈敏度：0.078 pg/mL
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反應時間：1-5h
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樣本體積：50-100ul
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檢測波長：450 nm
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研究領域：Signal Transduction
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測定原理：quantitative
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測定方法：Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度：
To assess the linearity of the assay, samples were spiked with high concentrations of rat gastrin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:100 Average % 90 Range % 85-95 1:200 Average % 94 Range % 90-98 1:400 Average % 99 Range % 95-104 1:800 Average % 102 Range % 98-107 -
回收率：
The recovery of rat gastrin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 92 88-96 EDTA plasma (n=4) 100 95-105 -
標準曲線：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
pg/ml OD1 OD2 Average Corrected 20 2.496 2.537 2.517 2.390 10 2.181 2.192 2.187 2.060 5 1.795 1.741 1.768 1.641 2.5 1.353 1.313 1.333 1.206 1.25 0.878 0.898 0.888 0.761 0.625 0.369 0.366 0.368 0.241 0.312 0.242 0.259 0.251 0.124 0 0.123 0.130 0.127 -
數據處理：
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貨期：3-5 working days
引用文獻
- Proteomic Insights into the Effects of Jianweixiaoshi Tablets on Functional Dyspepsia with Spleen Deficiency in Rats X Cheng, J Wan, D Sun, Y Zhan, J Yu, Y Li,Drug Design, Development and Therapy,2024
- Gastroprotective Effects of Spirulina platensis, Golden Kiwifruit Flesh, and Golden Kiwifruit Peel Extracts Individually or in Combination against Indomethacin-Induced Gastric Ulcer in Rats Ibrahim S. Aleid,Nutrients,2021
- The Key Ingredient Acacetin in Weishu Decoction Alleviates Gastrointestinal Motility Disorder Based on Network Pharmacology Analysis X Guo,Mediators of Inflammation,2021
- Regulation of gastrointestinal hormones during laxative activity of gallotannin-enriched extract isolated from Galla Rhois in loperamide-induced constipation of SD rats Kim JE, et al,Lab Anim Res,2018
- Glutinous rice amylopectin can adjust the plasma gut-regulated peptide levels in rhubarb-induced spleen deficiency rats. Zhang H.et al,Food Funct.,2016
產品評價
相關產品
靶點詳情
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功能：Gastrin stimulates the stomach mucosa to produce and secrete hydrochloric acid and the pancreas to secrete its digestive enzymes. It also stimulates smooth muscle contraction and increases blood circulation and water secretion in the stomach and intestine.
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基因功能參考文獻：
- we examined the chronic effects of a GLP-1-gastrin dual agonist ZP3022 on glycemic control and beta cell dysfunction in overtly diabetic Zucker Diabetic Fatty PMID: 25849341
- treatment with gastrin, a CCK2R agonist, stimulated the secretion of GLP-1, and that this effect was likely due to increased expression of proglucagon and PCSK1 (also known as prohormone convertase 3 (PC3 gene)). PMID: 25601282
- The results suggested that clusterin may contribute to gastrin's growth-promoting effect on the oxyntic mucosa. PMID: 21995960
- plays a role in the development of gastric mucosa, ECL cells and A-like cells in newborn and young rats PMID: 12220729
- role in increasing expression of COX-2 mRNA and protein, the activity of the COX-2 promoter, and the release of prostaglandin E(2) PMID: 12239223
- High temperature can affect gastrin, somatostatin and motilin production in the gastric antral mucosa of rats with gastric ulcer. PMID: 12376287
- study demonstrates that gastrin enhances gastric mucosal integrity through cyclooxygenase-2 PMID: 12388195
- Data suggest that distention of isolated rat stomach stimulates somatostatin release via cholinergic and non-cholinergic TTX-insensitive pathways, and that these pathways are responsible for distention-induced inhibition of gastrin release. PMID: 12508367
- gastrin induces Akt through activation of CCKB receptors and of intracellular calcium-dependent, PKC-independent, pathways PMID: 15351698
- the role of JAK-dependent pathways in the regulation of E-cadherin-dependent cell-cell adhesion by gastrin PMID: 15530849
- gastrin- and PACAP-stimulated secretion of histamine and pancreastatin is inhibited by somatostatin, misoprostol and galanin in enterochromaffin-like cells PMID: 15935492
- hypogastrinemia and impaired enterochromaffin cell function in the oxyntic mucosa of the stomach might be partially responsible for the reduction in body weight that occurs after gastric bypass PMID: 16645284
- G cells are luminal sampling chemomechanosensory cells whose gastrin secretion is regulated by neural, hormonal, luminal, and mechanical factors through protein kinase A activation, cAMP signaling, and mitogen-activated protein kinase phosphorylation. PMID: 19208342
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亞細胞定位：Secreted.
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蛋白家族：Gastrin/cholecystokinin family
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數據庫鏈接：
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