Rat Cytochrome P450 19A1(CYP19A1) ELISA kit

Rat Cytochrome P450 19A1(CYP19A1) ELISA kit

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中文名稱：

大鼠細胞色素P450 19A1(CYP19A1)酶聯免疫試劑盒
貨號：

CSB-EL006394RA
規格：

96T/48T
價格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產品描述：

大鼠細胞色素P450 19A1(CYP19A1)酶聯免疫試劑盒（CSB-EL006394RA）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的CYP19A1含量。CYP19A1是一種編碼芳香化酶的基因，位于細胞色素P450超家族，主要在肝臟和脂肪組織中表達。該酶在雌激素的生物合成中起關鍵作用，其活性異常與多種癌癥的發生發展密切相關。研究顯示，CYP19A1通過調控脂代謝和免疫應答在腫瘤中發揮作用，具有遺傳多樣性，其突變可能影響雌激素水平和細胞凋亡。試劑盒檢測范圍為18.75 pg/mL-1200 pg/mL，適用于體外分析大鼠內分泌調控機制、卵巢生理病理變化、環境污染物對激素代謝的影響等研究，為生殖生物學、毒理學及激素相關疾病模型的建立提供定量分析工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Cyp19a1 ELISA Kit; Arom ELISA Kit; Cyp19Aromatase ELISA Kit; EC 1.14.14.14 ELISA Kit; CYPXIX ELISA Kit; Cytochrome P-450AROM ELISA Kit; Cytochrome P450 19A1 ELISA Kit; Estrogen synthase ELISA Kit
縮寫：

CYP19A1
Uniprot No.：

P22443
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

18.75 pg/mL-1200 pg/mL
靈敏度：

4.68 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Metabolism
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat CYP19A1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	101
	Range %	95-107
1:2	Average %	91
	Range %	86-97
1:4	Average %	88
	Range %	84-92
1:8	Average %	95
	Range %	90-100

回收率：

The recovery of rat CYP19A1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	85	80-90
EDTA plasma (n=4)	96	91-103

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1200	2.452	2.272	2.362	2.237
600	1.705	1.832	1.769	1.644
300	1.286	1.316	1.301	1.176
150	0.829	0.880	0.855	0.730
75	0.504	0.469	0.487	0.362
37.5	0.301	0.285	0.293	0.168
18.75	0.203	0.211	0.207	0.082
0	0.127	0.122	0.125

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

Prochloraz Decreases Aromatase Activity and Serum Concentrations of E2, Testosterone, T3, and T4 in Pubertal Developmental Rats SY Lu,Taiwan Pesticide Science,2022
Tebuconazole Disrupted Aromatase Activity and Reduced 17β-Estradiol Concentration in Pubertal Developmental Rats SY Lu,Taiwan Pesticide Science,2020
PROTECTIVE EFFECTS OF CACTUS AND/OR PAPAYA JUICES AGAINST HEPATIC AND TESTICULAR TOXICITY INDUCED BY CHLORPYRIFOS IN ALBINO RATS Mogahed H E, et al,World Journal of Pharmaceutical Research,2019
Deltamethrin Alters Thyroid Hormones and Delays Pubertal Development in Male and Female Rats Pinpin Lin, et al,Comparative Endocrinology of Animals,2018

功能：

Catalyzes the formation of aromatic C18 estrogens from C19 androgens.
亞細胞定位：

Membrane; Peripheral membrane protein.
蛋白家族：

Cytochrome P450 family
數據庫鏈接：

STRING: 10116.ENSRNOP00000000212

UniGene: Rn.21402

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.