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中文名稱：大鼠半胱氨酸蛋白酶抑制劑/胱抑素C(Cys-C)酶聯免疫試劑盒
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貨號：CSB-E08385r
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規格：96T/48T
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價格：￥3900/￥2500
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其他：
產品詳情
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產品描述：大鼠半胱氨酸蛋白酶抑制劑/胱抑素C(Cys-C)酶聯免疫試劑盒（CSB-E08385r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、勻漿樣本中的CST3含量。CST3 即半胱氨酸蛋白酶抑制劑 C，是一種內源性半胱氨酸蛋白酶抑制劑。在人體生理中起穩定蛋白酶平衡等作用。研究發現其與神經系統疾病、腎臟疾病等密切相關，如可作為評估腎小球濾過功能指標，機制可能與調節體內蛋白酶活性和炎癥反應等有關。試劑盒檢測范圍為0.312 ng/ml-20 ng/ml，靈敏度為0.078 ng/ml。適用于科研領域中對腎臟疾病模型、藥物代謝研究或組織損傷相關實驗中Cys-C表達水平的動態監測，幫助研究者分析其與腎功能狀態、炎癥進程或細胞凋亡的關聯性。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
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別名：Cst3 ELISA Kit; Cystatin-C ELISA Kit; Cystatin-3 ELISA Kit
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縮寫：
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Uniprot No.：
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種屬：Rattus norvegicus (Rat)
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樣本類型：serum, plasma, homogenates
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檢測范圍：0.312 ng/ml-20 ng/ml
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靈敏度：0.078 ng/ml
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反應時間：1-5h
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樣本體積：50-100ul
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檢測波長：450 nm
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研究領域：Cardiovascular
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測定原理：quantitative
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測定方法：Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Intra-Assay Precision
Inter-Assay Precision
Sample
1
2
3
1
2
3
n
20
20
20
20
20
20
Mean(ng/ml)
2.490
2.550
2.588
2.467
2.527
2.555
SD
0.031
0.039
0.046
0.041
0.048
0.056
CV(%)
3.407
4.225
4.941
4.530
5.229
6.061
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線性度：
To assess the linearity of the assay, samples were spiked with high concentrations of rat Cys-C in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
Sample
Serum(n=4)
1:2
Average %
96
Range %
93-99
1:4
Average %
93
Range %
90-96
1:8
Average %
96
Range %
91-104
1:16
Average %
92
Range %
89-95
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回收率：
The recovery of rat Cys-C spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type
Average % Recovery
Range
Serum (n=5)
93
89-97
EDTA plasma (n=4)
94
90-98
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標準曲線：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml
OD1
OD2
Average
Corrected
20
2.564
2.587
2.576
2.404
10
1.923
1.934
1.929
1.757
5
1.329
1.368
1.349
1.177
2.5
0.912
0.937
0.925
0.753
1.25
0.582
0.598
0.590
0.418
0.625
0.412
0.436
0.424
0.252
0.312
0.311
0.317
0.349
0.177
0
0.170
0.174
0.172
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數據處理：
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貨期：3-5 working days
引用文獻
- Abatement by Alhagi maurorum of lead-induced nephrotoxicity in rats: emphasis on Nrf2/HO-1 MM Algandaby,Future Journal of Pharmaceutical Sciences,2025
- Sanqi oral solution ameliorates renal fibrosis by suppressing fibroblast activation via HIF-1α/PKM2/glycolysis pathway in chronic kidney disease D Hu, L Wang, Y Zhang, X Liu, Z Lu, H Li,Journal of Ethnopharmacology,2024
- Linagliptin mitigates cisplatin-induced kidney impairment via mitophagy regulation in rats, with emphasis on SIRT-3/PGC-1α, PINK-1 and Parkin-2 MGED Ewees, G Mostafa-Hadeab, S Saber,Toxicology and Applied Pharmacology,2024
- Clopidogrel protects against gentamicin-induced nephrotoxicity through targeting oxidative stress, apoptosis, and coagulation pathways AA Akila, RA Gad, MGED Ewees,Naunyn-Schmiedeberg's Archives of Pharmacology,2024
- Modulation of mi-RNA25/Ox-LDL/NOX4 signaling pathway by polyphenolic compound Hydroxytyrosol as a new avenue to alleviate cisplatin-induced acute kidney injury, a mechanistic study in rats MGED Ewees,Environmental toxicology and pharmacology,2023
- Rosuvastatin and diosmetin inhibited the HSP70/TLR4 /NF-κB p65/NLRP3 signaling pathways and switched macrophage to M2 phenotype in a rat model of acute kidney injury induced by cisplatin F Feng,Biomedicine & pharmacotherapy,2024
- Anandamide modulates WNT-5A/BCL-2, IP3/NFATc1, and HMGB1/NF-κB trajectories to protect against mercuric chloride-induced acute kidney injury DM Abdallah,Scientific reports,2023
- Effect of perinatal exposure to glyphosate and its mixture with 2, 4-D and dicamba on rat dam kidney and thyroid function and offspring's health AO Docea,Environmental research,2023
- 2-Methoxyestradiol TPGS Micelles Attenuate Cyclosporine A-Induced Nephrotoxicity in Rats through Inhibition of TGF-β1 and p-ERK1/2 Axis MW Al-Rabia,Antioxidants,2022
- Omeprazole Prevents Colistin-Induced Nephrotoxicity in Rats: Emphasis on Oxidative Stress, Inflammation, Apoptosis and Colistin Accumulation in Kidneys MZ Nasrullah,Pharmaceuticals,2022
- Renoprotective Effect of Vardenafil and Avanafil in Contrast-Induced Nephropathy: Emerging Evidence from an Animal Model IE Zisis,Journal of Personalized Medicine,2022
- Exogenous H2S Ameliorates High Salt-Induced Hypertension by Alleviating Oxidative Stress and Inflammation in the Paraventricular Nucleus in Dahl S Rats Yingying Liao,Cardiovascular Toxicology,2022
- Involvement of NOX-4/JAK/STAT pathway in the protective effect of aprepitant against diclofenac-induced renal toxicity HM Hafez,Life Sciences,2022
- Renoprotective effects of tropisetron through regulation of the TGF-β1, p53 and matrix metalloproteinases in streptozotocin-induced diabetic rats B Pourheydar,Chemico-Biological Interactions,2021
- The renoprotective effect of glycyrrhizic acid in insulin-resistant rats exposed to aluminum involves the inhibition of TLR4/NF-κB signaling pathway NA Emara,Naunyn-Schmiedeberg's Archives of Pharmacology,2020
- Protective Effects of Combination of Radix Astragali and Radix Salviae Miltiorrhizae on Kidney of Spontaneously Hypertensive Rats and Renal Intrinsic Cells Li W, et al,Chinese Journal of Integrative Medicine,2019
- Renoprotective effect of Zhenwu decoction against renal fibrosis by regulation of oxidative damage and energy metabolism disorder Li S.et al,Scientific Reports,2018
- Tempol, a superoxide dismutase mimetic agent, reduces cisplatin-induced nephrotoxicity in rats Mohamed G. Ewees.et al,drug and chemical toxicology,2018
- Ameliorative effect of ursolic acid on renal fibrosis in adenine-induced chronic kidney disease in rats RichaThakur.et al,Biomedicine & Pharmacotherapy,2018
- Stimulation of ACE2/ANG/Mas Axis by Diminazene Ameliorates Alzheimer's Disease in the D-Galactose-Ovariectomized Rat Model: Role of PI3K/Akt Kamel AS.et al,Mol Neurobiol,2018
- Nandrolone administration with or without strenuous exercise promotes overexpression of nephrin and podocin genes and induces structural and functional Tofighi A.et al,Toxicol Lett,2017
- Relevance of cystatin-C, N-acetylglucosaminidase, and Interleukin-18 with the diagnosis of acute kidney injury induced by cadmium in rats Mohamed Ali OS.et al,J Biochem Mol Toxicol.,2017
- Transplantation of insulin-producing cells to treat diabetic rats after 90% pancreatectomy Ya-Bin Yu. et al,World J Gastroenterol.,2015
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靶點詳情
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最新研究進展：CST3是一種由人體細胞產生的小分子蛋白質，也被稱為半胱氨酸蛋白酶抑制劑或淀粉樣蛋白沉積物抑制劑。它在細胞凋亡、免疫調節、組織修復和生殖系統發育等方面發揮重要作用。最近的研究表明，CST3與多種疾病有關，包括神經退行性疾病、腎臟疾病和腫瘤。
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功能：As an inhibitor of cysteine proteinases, this protein is thought to serve an important physiological role as a local regulator of this enzyme activity. Known to inhibit cathepsin B, H, and L.
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基因功能參考文獻：
- Tanshinone IIA protects against cardiac hypertrophy in spontaneously hypertensive rats by inhibiting the Cys-C/Wnt signaling pathway. PMID: 28053285
- These results indicate that Cd increases the urinary excretion of cystatin C, and they suggest that this effect may involve disruption of megalin-mediated uptake of cystatin C by epithelial cells of the proximal tubule. PMID: 26715107
- High cystatin C is associated with proteinuria. PMID: 21551085
- Triiodothyronine-induced increase in the production of cystatin C may be related to an increased cell metabolism and proteolysis control demand. PMID: 22360852
- CysC plays a protective role under conditions of neuronal challenge by inducing autophagy via mTOR inhibition PMID: 20352108
- Oxidants induce elevated cystatin C production from cardiac myocytes. Cystatin C plays a role in cardiac extracellular matrix remodelling. PMID: 20489058
- We found several novel glycoforms of cystatin c in rat hippocampal stem cells, suggesting that N- and O-linked glycans with sialic acid are attached to cystatin C. PMID: 15197734
- These results demonstrate that cystatin C has protective effects against various oxidative stresses that induce cell death. PMID: 16140167
- endometrial expression of the cysteine protease inhibitor, Cst3, is modulated by estrogen during the peri-implantation period PMID: 16935259
- These studies suggest alternate pathways and potential therapeutic targets of cell death associated with oxidative stress, CC, and lysosomal dysregulation. PMID: 17241700
- Finally, in vivo studies using kidney-specific megalin knockout mice provide evidence that megalin mediates proximal tubular uptake of cystatin C. We conclude that megalin is an endocytic receptor of cystatin C in PTC. PMID: 17462596
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亞細胞定位：Secreted.
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蛋白家族：Cystatin family
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