Rat Collagen Type Ⅲ,Col Ⅲ ELISA KIT

產(chǎn)品詳情

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

大鼠Ⅲ型膠原(Col Ⅲ)酶聯(lián)免疫試劑盒（CSB-E07924r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的Col Ⅲ含量。Col Ⅲ膠原蛋白是細(xì)胞外基質(zhì)的重要組成部分，參與組織結(jié)構(gòu)和功能。其研究背景涉及膠原蛋白在組織修復(fù)、纖維化疾病中的作用。研究機(jī)制主要圍繞Col Ⅲ的合成、降解及其與信號通路的關(guān)系，旨在揭示其在生理和病理過程中的作用。試劑盒檢測范圍為0.156 ng/mL-10 ng/mL，適用于實(shí)驗(yàn)室對大鼠纖維化疾病（如肝纖維化、肺纖維化）的機(jī)制研究、藥物干預(yù)效果評估或組織工程中膠原代謝的動態(tài)監(jiān)測，滿足科研場景中高通量、快速檢測的需求本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

Collagen Type 3,Col 3,_x000D_ Collagen Type III,Col III
縮寫：

Col Ⅲ
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.156 ng/mL-10 ng/mL
靈敏度：

0.039 ng/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat Col Ⅲ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	88-96
1:2	Average %	98
	Range %	92-104
1:4	Average %	95
	Range %	92-98
1:8	Average %	86
	Range %	80-94

回收率：

The recovery of rat Col Ⅲ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-98
EDTA plasma (n=4)	90	85-95

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

平均分:

5.0分 - 1 個(gè)評價(jià)

樣品類型：血清

樣品信息：大鼠

稀釋比：1:50

產(chǎn)品評價(jià)： RAT collagen type3, elisa試劑盒檢測大鼠血清，復(fù)孔的數(shù)據(jù)較穩(wěn)定。試劑盒溶液給量充足。此外，還發(fā)現(xiàn)該試劑盒可以檢測其他嚙齒動物的血清樣本，但OD值偏小。

By 杜老師