Rat Coagulation factor VII(F7) ELISA kit

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產品詳情

產品描述：

大鼠凝血因子VII(F7)酶聯免疫試劑盒（CSB-EL007930RA）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的F7含量。大鼠凝血因子VII（F7）是一種依賴維生素K的絲氨酸蛋白酶，在血液凝固的外部途徑中起關鍵作用。其與組織因子III（TF III）結合后，形成TF-FVIIa激活復合物，啟動凝血級聯反應，最終導致纖維蛋白凝塊形成。F7缺乏會導致圍產期死亡，因為其完全缺乏會導致正常血管中的出血。試劑盒檢測范圍為0.78 ng/mL-50 ng/mL，其水平變化與凝血功能異常、肝損傷及心血管疾病模型研究密切相關；支持科研領域對凝血機制、藥物作用靶點篩選、肝臟功能評估等研究需求本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

F7Coagulation factor VII ELISA Kit; EC 3.4.21.21 ELISA Kit; Serum prothrombin conversion accelerator) [Cleaved into: Factor VII light chain; Factor VII heavy chain] ELISA Kit
縮寫：

F7
Uniprot No.：

Q8K3U6
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.78 ng/mL-50 ng/mL
靈敏度：

0.195 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Blood Coagulation
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat F7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:200	Average %	91
	Range %	86-98
1:400	Average %	97
	Range %	92-106
1:800	Average %	93
	Range %	88-99
1:1600	Average %	89
	Range %	83-95

回收率：

The recovery of rat F7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-97
EDTA plasma (n=4)	109	103-115

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Initiates the extrinsic pathway of blood coagulation. Serine protease that circulates in the blood in a zymogen form. Factor VII is converted to factor VIIa by factor Xa, factor XIIa, factor IXa, or thrombin by minor proteolysis. In the presence of tissue factor and calcium ions, factor VIIa then converts factor X to factor Xa by limited proteolysis. Factor VIIa will also convert factor IX to factor IXa in the presence of tissue factor and calcium.
基因功能參考文獻：
1. The EGF(1) region of rat coagulation factor can mediate the specific binding of FVII with tissue factor. PMID: 18315926
亞細胞定位：

Secreted.
蛋白家族：

Peptidase S1 family
組織特異性：

Plasma.
數據庫鏈接：

KEGG: rno:260320

STRING: 10116.ENSRNOP00000038466

UniGene: Rn.86416