Rat Clara cell protein,CC16 ELISA Kit

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產(chǎn)品詳情

產(chǎn)品描述：

大鼠克拉拉細胞蛋白(CC16)酶聯(lián)免疫試劑盒（CSB-E08681r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的CC16含量。試劑盒檢測范圍為3.12 pg/mL-200 pg/mL，該產(chǎn)品適用于呼吸道炎癥模型、肺損傷機制研究、環(huán)境污染物暴露評估等科研領(lǐng)域，為探索CC16在肺部疾病發(fā)生發(fā)展中的作用、評估藥物治療效果或生物標志物篩選提供可靠工具，檢測數(shù)據(jù)可支持動物實驗中的病理生理學(xué)分析及分子機制研究本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

Scgb1a1 ELISA kit; Cc10 ELISA kit; Ugb ELISA kit; UtgUteroglobin ELISA kit; Clara cell phospholipid-binding protein ELISA kit; CCPBP ELISA kit; Clara cells 10 kDa secretory protein ELISA kit; CC10 ELISA kit; PCB-binding protein ELISA kit; Secretoglobin family 1A member 1 ELISA kit
縮寫：

CC16
Uniprot No.：

P17559
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

3.12 pg/mL-200 pg/mL
靈敏度：

0.78 pg/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Others
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat CC16 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	97
	Range %	92-102
1:2	Average %	91
	Range %	85-98
1:4	Average %	90
	Range %	88-93
1:8	Average %	100
	Range %	96-104

回收率：

The recovery of rat CC16 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	87-92
EDTA plasma (n=4)	106	103-110

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Binds phosphatidylcholine, phosphatidylinositol, polychlorinated biphenyls (PCB) and weakly progesterone, potent inhibitor of phospholipase A2.
基因功能參考文獻：
1. The expression level of CC16 in lung tissues and BALF decreased in rat lung tissues exposed to mixed air pollutants. PMID: 18303626
2. IL-13 induces CCSP expression via an EGFR- and leukocyte-dependent pathway. PMID: 12060562
3. Results show that secretory markers such as CFTR, CC10, and CC26 are present in taste cells of rat circumvallate papillae, and their immunoreactivity is expressed, to a different extent, in subsets of taste cells that express alpha-gustducin. PMID: 18156603
亞細胞定位：

Secreted.
蛋白家族：

Secretoglobin family
組織特異性：

Clara cells (nonciliated cells of the surface epithelium of the pulmonary airways).
數(shù)據(jù)庫鏈接：

KEGG: rno:25575

STRING: 10116.ENSRNOP00000027342

UniGene: Rn.2206