Rat Caspase-9,Casp-9 ELISA Kit

Rat Caspase-9,Casp-9 ELISA Kit

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中文名稱：

大鼠胱天蛋白酶9(Casp-9)酶聯免疫試劑盒
貨號：

CSB-E08863r
規格：

96T/48T
價格：

￥3900/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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引用文獻

產品描述：

大鼠胱天蛋白酶9(Casp-9)酶聯免疫試劑盒（CSB-E08863r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的Casp-9含量。Casp-9即半胱天冬酶9，在細胞凋亡機制中有重要背景。它是凋亡途徑關鍵成員，作為凋亡啟動者，被細胞色素C激活后，能招募相關蛋白形成復合物，激活下游Caspases級聯反應，引發細胞凋亡，是腫瘤等疾病研究的潛在靶點。試劑盒檢測范圍為62.5 pg/mL-4000 pg/mL，可廣泛應用于基礎科研領域，如凋亡信號通路機制研究、藥物開發中的靶點驗證或疾病模型中Caspase - 9活性變化的動態監測，為體外實驗提供穩定可靠的定量分析工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

Casp-9
Uniprot No.：

Q9JHK1
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

62.5 pg/mL-4000 pg/mL
靈敏度：

15.6 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cell Biology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat Casp-9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	96
	Range %	90-99
1:2	Average %	95
	Range %	91-102
1:4	Average %	98
	Range %	94-103
1:8	Average %	102
	Range %	96-106

回收率：

The recovery of rat Casp-9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-98
EDTA plasma (n=4)	100	96-104

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
4000	1.846	1.946	1.896	1.752
2000	1.442	1.542	1.492	1.348
1000	0.986	1.016	1.001	0.857
500	0.546	0.556	0.551	0.407
250	0.320	0.330	0.325	0.181
125	0.266	0.278	0.272	0.128
62.5	0.204	0.207	0.206	0.062
0	0.143	0.144	0.144

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.