Rat Carboxypeptidase B(CPB1) ELISA kit

產(chǎn)品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

大鼠羧肽酶B(CPB1)酶聯(lián)免疫試劑盒（CSB-EL005883RA）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的CPB1含量。CPB1，即羧肽酶B1，是一種胰腺特異性蛋白，參與蛋白質(zhì)消化。研究顯示，CPB1在胰腺癌全切術(shù)后表達(dá)上調(diào)，可能與疾病復(fù)發(fā)和轉(zhuǎn)移相關(guān)。研究機(jī)制涉及基因表達(dá)調(diào)控、蛋白質(zhì)功能分析等。試劑盒檢測范圍為12.5 ng/mL-800 ng/mL，適用于科研場景下對大鼠CPB1的表達(dá)水平分析，例如研究胰腺外分泌功能、營養(yǎng)代謝調(diào)控機(jī)制或建立相關(guān)疾病模型時的生物標(biāo)志物檢測，為探索CPB1在生理病理過程中的作用提供可靠工具本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

Cpb1 ELISA kit; CpbCarboxypeptidase B ELISA kit; EC 3.4.17.2 ELISA kit
縮寫：

CPB1
Uniprot No.：

P19223
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

12.5 ng/mL-800 ng/mL
靈敏度：

3.12 ng/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Metabolism
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat CPB1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	95-110
1:2	Average %	84
	Range %	80-88
1:4	Average %	98
	Range %	92-103
1:8	Average %	105
	Range %	100-112

回收率：

The recovery of rat CPB1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	84-93
EDTA plasma (n=4)	93	88-99

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.