Rat Apolipoprotein C-III(APOC3) ELISA kit

說明書下載

中文名稱：

大鼠載脂蛋白C-III(APOC3)酶聯(lián)免疫試劑盒
貨號(hào)：

CSB-EL001933RA
規(guī)格：

96T/48T
價(jià)格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產(chǎn)品描述：

大鼠載脂蛋白C-III(APOC3)酶聯(lián)免疫試劑盒（CSB-EL001933RA）為競(jìng)爭(zhēng)法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的APOC3含量。APOC3即載脂蛋白C3，它可抑制脂蛋白脂肪酶活性、阻礙肝臟對(duì)甘油三酯的攝取，與血漿甘油三酯水平密切相關(guān)。降低其水平可減少甘油三酯，目前圍繞它開發(fā)降血脂藥物是研究熱點(diǎn)，有望為心血管疾病防治提供新策略。試劑盒檢測(cè)范圍為31.25 ng/mL-2000 ng/mL，適用于脂代謝機(jī)制研究、藥物干預(yù)評(píng)價(jià)及疾病動(dòng)物模型（如高脂飲食誘導(dǎo)模型或基因修飾動(dòng)物）的病理分析，為心血管疾病、肥胖癥等領(lǐng)域的科研實(shí)驗(yàn)提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

Apoc3 ELISA Kit; Apolipoprotein C-III ELISA Kit; Apo-CIII ELISA Kit; ApoC-III ELISA Kit; Apolipoprotein C3 ELISA Kit
縮寫：

APOC3
Uniprot No.：

P06759
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates
檢測(cè)范圍：

31.25 ng/mL-2000 ng/mL
靈敏度：

7.8 ng/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Metabolism
測(cè)定原理：

quantitative
測(cè)定方法：

Competitive

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat APOC3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	92
	Range %	88-96
1:200	Average %	95
	Range %	91-99
1:400	Average %	93
	Range %	90-97
1:800	Average %	95
	Range %	89-99

回收率：

The recovery of rat APOC3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	87-93
EDTA plasma (n=4)	87	84-90

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average
2000	0.093	0.089	0.091
1000	0.152	0.144	0.148
500	0.227	0.218	0.223
250	0.336	0.328	0.332
125	0.498	0.488	0.493
62.5	0.657	0.640	0.649
31.25	0.963	0.893	0.928
0	1.673	1.583	1.628

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

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功能：

Component of triglyceride-rich very low density lipoproteins (VLDL) and high density lipoproteins (HDL) in plasma. Plays a multifaceted role in triglyceride homeostasis. Intracellularly, promotes hepatic very low density lipoprotein 1 (VLDL1) assembly and secretion; extracellularly, attenuates hydrolysis and clearance of triglyceride-rich lipoproteins (TRLs). Impairs the lipolysis of TRLs by inhibiting lipoprotein lipase and the hepatic uptake of TRLs by remnant receptors. Formed of several curved helices connected via semiflexible hinges, so that it can wrap tightly around the curved micelle surface and easily adapt to the different diameters of its natural binding partners.
亞細(xì)胞定位：

Secreted.
蛋白家族：

Apolipoprotein C3 family
組織特異性：

Synthesized predominantly in liver and to a lesser degree in intestine.
數(shù)據(jù)庫鏈接：

STRING: 10116.ENSRNOP00000067045

UniGene: Rn.129547

Rat Apolipoprotein C-III(APOC3) ELISA kit

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