Rat 6-keto-PGF1α ELISA Kit

Rat 6-keto-PGF1α ELISA Kit

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中文名稱：

大鼠6-酮前列腺素F1α(6-keto-PGF1α)酶聯(lián)免疫試劑盒
貨號(hào)：

CSB-E14411r
規(guī)格：

96T/48T
價(jià)格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產(chǎn)品描述：

大鼠6-酮前列腺素F1α(6-keto-PGF1α)酶聯(lián)免疫試劑盒（CSB-E14411r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、組織培養(yǎng)上清液樣本中的6-keto-PGF1α含量。6-keto-PGF1α是一種由PGI2代謝生成的非活性產(chǎn)物，作為PGI2生物合成的有用標(biāo)志物。其研究機(jī)制涉及HIF-1α、TXB2、6-Keto-PGF1α和HA的聯(lián)合檢測，以反映肝臟微循環(huán)障礙狀況，其在慢性乙型肝炎等疾病診斷中具有重要意義。試劑盒檢測范圍為31.25 pg/mL-2000 pg/mL，常用于研究血管舒縮調(diào)節(jié)、炎癥反應(yīng)及血栓形成等生理病理過程；支持心血管疾病模型、藥物干預(yù)實(shí)驗(yàn)及炎癥機(jī)制等基礎(chǔ)研究，尤其適用于探索氧化應(yīng)激、內(nèi)皮損傷或抗血小板藥物藥效評(píng)價(jià)等領(lǐng)域；通過檢測不同樣本中6 - keto - PGF1α的動(dòng)態(tài)變化，可為血管功能相關(guān)科研課題提供可靠數(shù)據(jù)支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

6-keto-PGF1α,6-keto-PGF1 alpha
縮寫：

6-keto-PGF1α
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates, cell culture supernates
檢測范圍：

31.25 pg/mL-2000 pg/mL
靈敏度：

7.81 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Cardiovascular
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat 6-keto-PGF1α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	85
	Range %	80-90
1:2	Average %	88
	Range %	82-95
1:4	Average %	90
	Range %	85-95
1:8	Average %	92
	Range %	84-97

回收率：

The recovery of rat 6-keto-PGF1α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-100
EDTA plasma (n=4)	98	93-103

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2000	2.151	2.105	2.128	2.036
1000	1.744	1.659	1.702	1.610
500	1.328	1.294	1.311	1.219
250	0.972	0.896	0.934	0.842
125	0.627	0.602	0.615	0.523
62.5	0.313	0.289	0.301	0.209
31.25	0.196	0.191	0.194	0.102
0	0.095	0.088	0.092

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.