Rat α2 macroglobulin,α2 MG ELISA Kit

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產品詳情

產品描述：

大鼠α2巨球蛋白(α2-MG)酶聯免疫試劑盒（CSB-E13863r）為競爭法ELISA試劑盒，定量檢測血清、血漿樣本中的A2M含量。A2M（α-2-巨球蛋白）是一種在血漿中含量豐富的糖蛋白，具有蛋白酶抑制作用，參與急性炎癥反應。其研究機制涉及與多種免疫炎癥因子結合，調節體內小分子平衡。在神經系統疾病中，A2M與阿爾茨海默病、多發性硬化等疾病相關，可能通過空間捕獲蛋白酶來抑制其活性。試劑盒檢測范圍為3.13 ng/mL-800 ng/mL，該產品專為科研領域設計，可應用于炎癥機制研究、肝腎功能評估、疾病動物模型分析及藥物干預效果評價等方向，為探索α2 - MG在病理生理過程中的作用提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

A2m ELISA kit; A2m1Alpha-2-macroglobulin ELISA kit; Alpha-2-M ELISA kit
縮寫：

A2M
Uniprot No.：

P06238
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma
檢測范圍：

3.13 ng/mL-800 ng/mL
靈敏度：

3.125 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Competitive

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat α2 MG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1000	Average %	97
	Range %	88-105
1:2000	Average %	91
	Range %	89-102
1:4000	Average %	93
	Range %	90-98
1:8000	Average %	94
	Range %	87-98

回收率：

The recovery of rat α2 MG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	92-102
EDTA plasma (n=4)	95	90-99

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.
亞細胞定位：

Secreted.
蛋白家族：

Protease inhibitor I39 (alpha-2-macroglobulin) family
組織特異性：

Highest constitutive expression in ovary. Low level in testis, uterus and non-acute phase liver. Protein found in plasma.
數據庫鏈接：

KEGG: rno:100911545

STRING: 10116.ENSRNOP00000019346

UniGene: Rn.225884