Rabbit soluble P-selectin,sP-selectin ELISA Kit

產品詳情

文獻引用 (2) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

兔子可溶性P選擇素(sP-selectin)酶聯免疫試劑盒（CSB-E06861Rb）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織培養上清液、尿液、唾液、組織勻漿樣本中的sP-selectin含量。sP - selectin是一個受關注的靶點。它在炎癥、血栓形成等病理生理過程中發揮重要作用。相關研究主要聚焦其介導細胞粘附、調節免疫反應等機制，通過抑制或調控它的活性，有望為治療炎癥性疾病、心血管疾病等開拓新的方向。試劑盒檢測范圍為31.25 pg/mL-2000 pg/mL，可滿足基礎科研中疾病機制研究、藥物作用評估或生物標志物探索等需求，尤其適用于炎癥模型、血管功能實驗或代謝相關研究的體外樣本檢測。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

sP-selectin
種屬：

Oryctolagus cuniculus (Rabbit)
樣本類型：

serum, plasma, cell culture supernates, urine, saliva, tissue homogenates
檢測范圍：

31.25 pg/mL-2000 pg/mL
靈敏度：

7.8 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Others
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit sP-selectin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:20	Average %	98
	Range %	95-101
1:40	Average %	96
	Range %	93-99
1:80	Average %	87
	Range %	85-90
1:160	Average %	92
	Range %	87-95

回收率：

The recovery of rabbit sP-selectin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	93-99
EDTA plasma (n=4)	101	99-104

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.