Rabbit interleukin 12 (IL-12/P70) ELISA kit

產品詳情

文獻引用 (2) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

兔白介素12(IL-12/P70)酶聯免疫試劑盒（CSB-EQ027628RB）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的IL12/P70含量。IL12/P70是重要的免疫靶點。它由p35和p40亞基組成，在調節免疫細胞功能、促進Th1細胞分化等方面發揮關鍵作用。其研究機制主要聚焦于調控免疫應答相關信號通路。深入研究它有望為免疫相關疾病治療提供新策略。試劑盒檢測范圍為3.12 pg/mL-200 pg/mL，該產品適用于實驗動物模型的免疫調控研究、感染性疾病機制探索、疫苗效力評估及炎癥相關科研項目，為研究IL-12/P70在免疫微環境中的作用提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

IL12/P70, Interleukin-12/P70
縮寫：

IL-12/P70
種屬：

Oryctolagus cuniculus (Rabbit)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

3.12 pg/mL-200 pg/mL
靈敏度：

0.78 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit IL-12 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	89-95
1:2	Average %	90
	Range %	83-99
1:4	Average %	95
	Range %	92-102
1:8	Average %	93
	Range %	86-99

回收率：

The recovery of rabbit IL-12 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	86-110
EDTA plasma (n=4)	97	89-99

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.