Rabbit creatine kinase MB isoenzyme (CK-MB) ELISA kit

產(chǎn)品詳情

說(shuō)明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

兔肌酸激酶同工酶MB(CK-MB)酶聯(lián)免疫試劑盒（CSB-EQ027653RB）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的CK-MB含量。CK-MB即肌酸激酶同工酶，主要存在于心肌中。當(dāng)心肌損傷時(shí)，CK - MB會(huì)釋放入血，其水平變化可反映心肌損傷程度。研究多圍繞其作為心肌損傷早期敏感標(biāo)志物展開，監(jiān)測(cè)它對(duì)急性心肌梗死等疾病的診斷、治療及預(yù)后評(píng)估有重要意義。試劑盒檢測(cè)范圍為1.56 mU/mL-100 mU/mL，適用于心血管疾病機(jī)制研究、藥物心臟毒性評(píng)估及動(dòng)物模型中心肌損傷的動(dòng)態(tài)監(jiān)測(cè)；適用于心肌缺血再灌注損傷、藥物干預(yù)效果評(píng)價(jià)、病理模型建立等科研場(chǎng)景，助力心血管相關(guān)疾病的基礎(chǔ)研究與轉(zhuǎn)化探索本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說(shuō)明書。
縮寫：

CK-MB
種屬：

Oryctolagus cuniculus (Rabbit)
樣本類型：

serum, plasma, tissue homogenates
檢測(cè)范圍：

1.56 mU/mL-100 mU/mL
靈敏度：

0.39 mU/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Cardiovascular
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit CK-MB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	89
	Range %	84-93
1:2	Average %	95
	Range %	90-99
1:4	Average %	94
	Range %	89-100
1:8	Average %	102
	Range %	96-107

回收率：

The recovery of rabbit CK-MB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	88	83-96
EDTA plasma (n=4)	92	88-98

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.