Rabbit Pulmonary surfactant-associated protein B(SFTPB) ELISA kit

產(chǎn)品詳情

說(shuō)明書(shū)下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

兔肺表面活性物質(zhì)相關(guān)蛋白B(SFTPB)酶聯(lián)免疫試劑盒（CSB-EL021173RB）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的SFTPB含量。試劑盒檢測(cè)范圍為1.87 ng/mL-120 ng/mL，該產(chǎn)品適用于呼吸生理學(xué)研究，可探究SFTPB在肺損傷修復(fù)、感染性肺炎或遺傳性肺泡蛋白沉積癥等動(dòng)物模型中的動(dòng)態(tài)變化，也可用于評(píng)估肺表面活性物質(zhì)代謝調(diào)控機(jī)制或藥物干預(yù)效果。支持血清、血漿直接檢測(cè)及肺組織等勻漿樣本處理，滿(mǎn)足不同實(shí)驗(yàn)設(shè)計(jì)的科研需求。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
別名：

SFTPB ELISA Kit; SFTP3 ELISA Kit; Pulmonary surfactant-associated protein B ELISA Kit; SP-B ELISA Kit; 6 kDa protein ELISA Kit; Pulmonary surfactant-associated proteolipid SPL(Phe) ELISA Kit
縮寫(xiě)：

SFTPB
Uniprot No.：

P15285
種屬：

Oryctolagus cuniculus (Rabbit)
樣本類(lèi)型：

serum, plasma, tissue homogenates
檢測(cè)范圍：

1.87 ng/mL-120 ng/mL
靈敏度：

0.46 ng/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Signal Transduction
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit SFTPB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	87
	Range %	82-93
1:2	Average %	95
	Range %	91-99
1:4	Average %	94
	Range %	89-98
1:8	Average %	89
	Range %	86-96

回收率：

The recovery of rabbit SFTPB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	86-98
EDTA plasma (n=4)	88	82-94

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Pulmonary surfactant-associated proteins promote alveolar stability by lowering the surface tension at the air-liquid interface in the peripheral air spaces. SP-B increases the collapse pressure of palmitic acid to nearly 70 millinewtons per meter.
亞細(xì)胞定位：

Secreted, extracellular space, surface film.
數(shù)據(jù)庫(kù)鏈接：

KEGG: ocu:100009194

STRING: 9986.ENSOCUP00000025658

UniGene: Ocu.1963