Rabbit Pulmonary surfactant-associated protein A(SFTPA1) ELISA kit

產品詳情

文獻引用 (1) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

兔肺表面活性物質相關蛋白A(SFTPA1)酶聯免疫試劑盒（CSB-EL021168RB）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的SFTPA1含量。SFTPA1，即肺表面活性物質相關蛋白A，是一種在肺泡II型上皮細胞中合成的蛋白質，主要功能是降低肺泡表面張力，維持肺泡穩定性。其研究涉及肺泡生理、病理及疾病治療等領域。試劑盒檢測范圍為1.56 ng/mL-100 ng/mL，適用于科研領域中對兔呼吸道疾病模型的機制研究，如評估肺損傷修復過程中 SFTPA1 的動態變化，探究感染或炎癥狀態下肺泡蛋白的調控規律，或用于藥物干預實驗中生物標志物的定量分析。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

SFTPA1 ELISA Kit; SFTP1 ELISA Kit; SFTPA ELISA Kit; Pulmonary surfactant-associated protein A ELISA Kit; PSAP ELISA Kit; PSP-A ELISA Kit; SP-A ELISA Kit
縮寫：

SFTPA1
Uniprot No.：

P12842
種屬：

Oryctolagus cuniculus (Rabbit)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

1.56 ng/mL-100 ng/mL
靈敏度：

0.39 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit SFTPA1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	82-96
1:2	Average %	107
	Range %	90-111
1:4	Average %	90
	Range %	85-94
1:8	Average %	91
	Range %	85-95

回收率：

The recovery of rabbit SFTPA1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	82-95
EDTA plasma (n=4)	99	90-105

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

In presence of calcium ions, it binds to surfactant phospholipids and contributes to lower the surface tension at the air-liquid interface in the alveoli of the mammalian lung and is essential for normal respiration. Enhances the expression of MYO18A/SP-R210 on alveolar macrophages.
亞細胞定位：

Secreted, extracellular space, extracellular matrix. Secreted, extracellular space, surface film.
蛋白家族：

SFTPA family
數據庫鏈接：

KEGG: ocu:100009044

STRING: 9986.ENSOCUP00000012830

UniGene: Ocu.1962