Rabbit Osteocalcin/bone gla protein,OT/BGP ELISA Kit

產品詳情

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文獻引用 (1) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

兔子骨鈣素/骨谷氨酸蛋白(OT/BGP)酶聯免疫試劑盒（CSB-E14060Rb）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的BGLAP含量。BGLAP即骨鈣素，是骨組織中含量豐富的非膠原蛋白。其背景在于它是成骨細胞特異性分泌的，反映骨代謝狀態。研究機制方面，它不僅參與骨礦化和骨轉換，還在能量代謝、生殖等系統有調節作用，為多領域疾病研究提供潛在靶點。試劑盒檢測范圍為1.25 ng/mL-80 ng/mL，常用于骨代謝調控機制、骨質疏松癥模型評估及骨骼相關藥物開發等研究。適用于體外分析骨組織工程材料對成骨細胞功能的影響、疾病模型中骨代謝動態監測、以及骨相關生物標志物的基礎研究。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

BGLAP ELISA Kit; Osteocalcin ELISA Kit; Bone Gla protein ELISA Kit; BGP ELISA Kit; Gamma-carboxyglutamic acid-containing protein ELISA Kit
縮寫：

BGLAP
Uniprot No.：

P39056
種屬：

Oryctolagus cuniculus (Rabbit)
樣本類型：

serum, plasma, tissue homogenates, cell lysates
檢測范圍：

1.25 ng/mL-80 ng/mL
靈敏度：

0.31 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Metabolism
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit OT/BGP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	87
	Range %	82-93
1:2	Average %	95
	Range %	89-99
1:4	Average %	94
	Range %	90-90
1:8	Average %	94
	Range %	86-98

回收率：

The recovery of rabbit OT/BGP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	90-94
EDTA plasma (n=4)	92	87-96

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium.
亞細胞定位：

Secreted.
蛋白家族：

Osteocalcin/matrix Gla protein family
數據庫鏈接：

STRING: 9986.ENSOCUP00000020318