Rabbit Krebs yon den Lundgen-6 (KL-6) ELISA Kit

產品詳情

文獻引用 (1) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

兔子Ⅱ型肺泡細胞表面抗原(KL-6)酶聯免疫試劑盒（CSB-E14112Rb）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的KL-6含量。KL-6，也稱為涎液化糖鏈抗原，是一種表達于II型肺泡上皮細胞的糖蛋白，被認為是診斷間質性肺疾病（ILD）的準確性最高的生物標記物。其表達增多與肺泡上皮細胞增生和肺泡基底膜損傷有關，在ILD的早期診斷和病情評估中具有重要價值。試劑盒檢測范圍為50 IU/mL-800 IU/mL，支持科研人員在體外實驗中定量評估肺損傷模型、藥物干預研究或疾病生物標志物探索中的 KL - 6 動態變化，為呼吸系統疾病的基礎研究提供靈敏可靠的工具本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

KL-6
Uniprot No.：
種屬：

Oryctolagus cuniculus (Rabbit)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

50 IU/mL-800 IU/mL
靈敏度：

25 IU/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Others
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit KL-6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	87
	Range %	82-93
1:2	Average %	94
	Range %	89-99
1:4	Average %	92
	Range %	87-96
1:8	Average %	95
	Range %	90-100

回收率：

The recovery of rabbit KL-6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	91-99
EDTA plasma (n=4)	89	87-93

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.