Rabbit Interleukin 2,IL-2 ELISA Kit

產品詳情

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文獻引用 (3) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

兔子白介素2(IL-2)酶聯免疫試劑盒（CSB-E08745Rb）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿樣本中的IL2含量。IL2是重要的細胞因子，在免疫調節中發揮關鍵作用。其背景上，是免疫系統抵御疾病的重要介質。研究機制方面，IL2可激活T細胞和NK細胞等免疫細胞，增強其殺傷活性，在腫瘤免疫治療、自身免疫病等研究領域被廣泛關注。試劑盒檢測范圍為31.25 pg/mL-2000 pg/mL，適用于動物模型研究、免疫機制探索、炎癥相關疾病機制分析或疫苗效力評估等科研場景，為探究IL - 2在免疫系統中的調控作用提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

IL2Interleukin-2 ELISA Kit; IL-2 ELISA Kit; T-cell growth factor ELISA Kit; TCGF ELISA Kit
縮寫：

IL2
Uniprot No.：

O77620
種屬：

Oryctolagus cuniculus (Rabbit)
樣本類型：

serum, plasma
檢測范圍：

31.25 pg/mL-2000 pg/mL
靈敏度：

7.8 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit IL-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	87
	Range %	82-93
1:2	Average %	95
	Range %	91-100
1:4	Average %	86
	Range %	80-95
1:8	Average %	93
	Range %	82-98

回收率：

The recovery of rabbit IL-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	80-98
EDTA plasma (n=4)	93	84-100

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells.
基因功能參考文獻：
1. IL-2 activates the STAT3 pathway, protects the lens epithelium cells and reduce the damage caused by inflammation reactions. PMID: 18201527
亞細胞定位：

Secreted.
蛋白家族：

IL-2 family
數據庫鏈接：

KEGG: ocu:100302458

STRING: 9986.ENSOCUP00000008701

UniGene: Ocu.2461