Rabbit Interleukin 18,IL-18 ELISA Kit

產品詳情

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文獻引用 (1) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

兔子白介素18(IL-18)酶聯免疫試劑盒（CSB-E13942Rb）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿樣本中的IL-18含量。IL-18是一個重要靶點。它屬于白細胞介素家族，在免疫調節中發揮關鍵作用。研究發現，IL-18參與多種炎癥相關疾病發生發展。其作用機制主要是通過結合受體激活下游信號通路，調控免疫細胞功能，為相關疾病治療提供了新方向。試劑盒檢測范圍為12.5 pg/mL-800 pg/mL，適用于科研場景中IL - 18濃度的精準分析，例如免疫機制研究、炎癥動物模型評估或疫苗開發中免疫原性評價。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

IL-18
Uniprot No.：

B1A3U4
種屬：

Oryctolagus cuniculus (Rabbit)
樣本類型：

serum, plasma
檢測范圍：

12.5 pg/mL-800 pg/mL
靈敏度：

3.12 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit IL-18 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	86-95
1:2	Average %	102
	Range %	97-107
1:4	Average %	91
	Range %	85-97
1:8	Average %	97
	Range %	91-103

回收率：

The recovery of rabbit IL-18 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-98
EDTA plasma (n=4)	102	92-107

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.