Rabbit C-Reactive Protein,CRP ELISA Kit

產(chǎn)品詳情

文獻引用 (6) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

兔子C反應(yīng)蛋白(CRP)酶聯(lián)免疫試劑盒（CSB-E06847Rb）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿樣本中的CRP含量。CRP即C反應(yīng)蛋白，是一種急性時相反應(yīng)蛋白。其背景在于機體受感染或組織損傷時，血漿中濃度急劇升高。研究機制方面，它可激活補體系統(tǒng)、調(diào)節(jié)淋巴細胞功能等，在炎癥反應(yīng)、免疫調(diào)節(jié)等過程中發(fā)揮重要作用，是臨床常用的炎癥指標(biāo)。試劑盒檢測范圍為7.8 pg/mL-2000 pg/mL，動物模型中的免疫調(diào)控機制研究、炎癥相關(guān)疾病模型評估、藥物干預(yù)效果分析、實驗性關(guān)節(jié)炎模型、感染性疾病模型或代謝綜合征研究中血清/血漿 CRP 的動態(tài)監(jiān)測、探索炎癥通路機制及潛在治療靶點本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

CRP ELISA kit; PTX1C-reactive protein ELISA kit
縮寫：

CRP
Uniprot No.：

P02742
種屬：

Oryctolagus cuniculus (Rabbit)
樣本類型：

serum, plasma
檢測范圍：

7.8 pg/mL-2000 pg/mL
靈敏度：

2 pg/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit CRP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1000	Average %	96
	Range %	90-102
1:2000	Average %	91
	Range %	82-96
1:4000	Average %	90
	Range %	85-98
1:8000	Average %	94
	Range %	83-98

回收率：

The recovery of rabbit CRP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	90-104
EDTA plasma (n=4)	91	85-96

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells.
基因功能參考文獻：
1. Rabbit CRP acute-phase system binds phosphocholine and phosphoethanolamine. PMID: 23600950
2. IL-6 augments mechanical strain-induced CRP synthesis in vessels via the stretch-activated channel-NF-kappaB pathway. PMID: 23257175
3. CRP antagonism of eNOS is mediated by coupling of FcgammaRI to FcgammaRIIB by Src kinase and activation of inositol 5'-phosphatase 1, and consistent with this mechanism, both FcgammaRI and FcgammaRIIB are required for CRP to blunt endothelial repair in vivo. PMID: 21940940
4. Metformin attenuated the progression of aortic atherosclerosis via down-regulation of serum C-reactive protein. PMID: 20108078
5. During abdominal aortic aneurysm formation, increased serum hsCRP levels derive from aneurysmal arteries with degenerating elastic lamina. PMID: 19502992
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亞細胞定位：

Secreted.
蛋白家族：

Pentraxin family
組織特異性：

Found in plasma.
數(shù)據(jù)庫鏈接：

KEGG: ocu:100009091

STRING: 9986.ENSOCUP00000004059

UniGene: Ocu.1848