Pig tissue inhibitors of metalloproteinase 1,TIMP-1 ELISA Kit

Pig tissue inhibitors of metalloproteinase 1,TIMP-1 ELISA Kit

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中文名稱：

豬基質(zhì)金屬蛋白酶抑制因子1(TIMP-1)酶聯(lián)免疫試劑盒
貨號：

CSB-E06799p
規(guī)格：

96T/48T
價(jià)格：

￥4200/￥3000
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產(chǎn)品描述：

豬基質(zhì)金屬蛋白酶抑制因子1(TIMP-1)酶聯(lián)免疫試劑盒（CSB-E06799p）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的TIMP1含量。TIMP1即金屬蛋白酶組織抑制因子1，它在維持細(xì)胞外基質(zhì)穩(wěn)態(tài)中起關(guān)鍵作用。其表達(dá)異常與腫瘤發(fā)生、轉(zhuǎn)移及預(yù)后等密切相關(guān)。研究機(jī)制上，TIMP1可抑制基質(zhì)金屬蛋白酶活性，還能通過多種信號通路影響細(xì)胞增殖、凋亡等生物學(xué)行為。試劑盒檢測范圍為7.8 ng/mL-500 ng/mL，適用于心血管疾病、肝臟纖維化、腫瘤微環(huán)境等領(lǐng)域的科研探索，為研究組織再生機(jī)制、炎癥相關(guān)信號通路或疾病模型中的基質(zhì)重塑提供定量工具，滿足體外實(shí)驗(yàn)中對TIMP - 1蛋白表達(dá)水平的動(dòng)態(tài)監(jiān)測需求。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

TIMP1 ELISA Kit; Metalloproteinase inhibitor 1 ELISA Kit; Tissue inhibitor of metalloproteinases 1 ELISA Kit; TIMP-1 ELISA Kit
縮寫：

TIMP1
Uniprot No.：

P35624
種屬：

Sus scrofa (Pig)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

7.8 ng/mL-500 ng/mL
靈敏度：

1.95 ng/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Cardiovascular
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig TIMP-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	89-101
1:2	Average %	89
	Range %	83-98
1:4	Average %	92
	Range %	86-105
1:8	Average %	94
	Range %	89-107

回收率：

The recovery of pig TIMP-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	85-99
EDTA plasma (n=4)	97	92-100

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
500	2.422	2.395	2.409	2.236
250	1.466	1.506	1.486	1.313
125	0.986	1.007	0.997	0.824
62.5	0.611	0.589	0.600	0.427
31.2	0.425	0.454	0.440	0.267
15.6	0.342	0.362	0.352	0.179
7.8	0.242	0.250	0.246	0.073
0	0.175	0.170	0.173

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

■ 常見問題解答

Regarding your ELISA kit CSB-E06799p, could you please advise on the sample types that you lab has tested with this kit, and what the observed concentration values were?
I was hoping to use their porcine cartilage homogenate samples with the kit. I have already homogenized the samples in RIPA buffer, and I was hoping you might be able to advise whether or not you thought RIPA might be a suitable lysis buffer for my samples?

Thanks for your inquiry.
CSB-E06799p
We tested undiluted serum and plasma samples before and the test value is ND--30ng/ml. Pls find attached QC.
For cartilage samples, sorry we didn't use RIPA buffer before. Usually we use pretreatment combined with repeated freezing and thawing method.
Generally, RIPA will contain components such as SDS, Triton X-100, and NP-40. Pls note:
1. It must not contain SDS (which will destroy the hydrogen bond of protein macromolecules causing protein denaturation);
2. For Triton X-100, NP 40 and other mild detergents, you should control the low concentration range (generally no more than 1%, but it not verified, so we are not clear about the specific impact);
3.EGTA and EDTA can be used as chelating agents for metal ions to remove metal ions, which has a great influence on BCA method, but we are not clear about the impact on elisa;
4 The sample should not contain NaN3 components, which will inhibit HRP activity and cause false negative.
Therefore, if your sample has been prepared, you can do a pretest first but we are not clear about the final test result; if not prepared, it is recommended to follow the recommended method in our manual.
Pls let me know if you have any further questions. Thank you.

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功能：

Metalloproteinase inhibitor that functions by forming one to one complexes with target metalloproteinases, such as collagenases, and irreversibly inactivates them by binding to their catalytic zinc cofactor. Acts on MMP1, MMP2, MMP3, MMP7, MMP8, MMP9, MMP10, MMP11, MMP12, MMP13 and MMP16. Does not act on MMP14. Also functions as a growth factor that regulates cell differentiation, migration and cell death and activates cellular signaling cascades via CD63 and ITGB1. Plays a role in integrin signaling.
基因功能參考文獻(xiàn)：
1. Results provide evidence for the utility of MMP9 and TIMP1 as markers of age- and lactocrine-sensitive porcine female reproductive tract development. PMID: 27866058
2. we demonstrated the presence of high molecular weight (HMW) complexes (130, 170, and 220 kDa) containing MMP9, TIMP1, and NGAL (also MMP2 in 220 kDa complex) without proteolytic activity. PMID: 23710440
3. Hemodialysis graft placement leads to early increases in wall shear stress, VEGF-A, pro-MMP-9, MMP-2, VEGFR-1, VEGFR-2, and TIMP-1, which may contribute to the development of venous stenosis. PMID: 18326810
4. Results indicate that leukemia inhibitory factor (LIF) and Oncostatin M increase the expression of MMP-1, MMP-3, and TIMP-1 several fold, and that their expression is reduced to basal levels in the presence of the LIF antagonist MH35-BD. PMID: 19342253
亞細(xì)胞定位：

Secreted.
蛋白家族：

Protease inhibitor I35 (TIMP) family
數(shù)據(jù)庫鏈接：

KEGG: ssc:396862

STRING: 9823.ENSSSCP00000013066

UniGene: Ssc.11784

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.