Pig serum amyloid A,SAA ELISA Kit

產(chǎn)品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

豬血清淀粉樣蛋白A(SAA)酶聯(lián)免疫試劑盒（CSB-E13309p）為競(jìng)爭(zhēng)法ELISA試劑盒，定量檢測(cè)血清、血漿樣本中的SAA含量。試劑盒檢測(cè)范圍為19.53 pg/mL-5000 pg/mL，適用于科研領(lǐng)域中對(duì)豬免疫應(yīng)答機(jī)制、感染性疾病模型或疫苗效果評(píng)估等研究。通過精確檢測(cè)SAA水平，研究者可動(dòng)態(tài)監(jiān)測(cè)實(shí)驗(yàn)動(dòng)物炎癥反應(yīng)進(jìn)程，為畜牧養(yǎng)殖健康管理、病理機(jī)制探索及藥物干預(yù)效果分析提供數(shù)據(jù)支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

SAA2; Serum amyloid A-2 protein
縮寫：

SAA
Uniprot No.：

P0DSO0
種屬：

Sus scrofa (Pig)
樣本類型：

serum, plasma
檢測(cè)范圍：

19.53 pg/mL-5000 pg/mL
靈敏度：

19.53 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Cardiovascular
測(cè)定原理：

quantitative
測(cè)定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig SAA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1000	Average %	97
	Range %	92-103
1:2000	Average %	95
	Range %	91-99
1:4000	Average %	101
	Range %	98-105
1:8000	Average %	95
	Range %	86-96

回收率：

The recovery of pig SAA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	88-99
EDTA plasma (n=4)	103	98-107

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.