Pig oxidized low density lipoprotein,OxLDL ELISA Kit

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中文名稱：

豬氧化低密度脂蛋白(OxLDL)酶聯(lián)免疫試劑盒
貨號(hào)：

CSB-E06527p
規(guī)格：

96T/48T
價(jià)格：

￥4200/￥3000
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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豬氧化低密度脂蛋白(OxLDL)酶聯(lián)免疫試劑盒（CSB-E06527p）為競(jìng)爭(zhēng)法ELISA試劑盒，定量檢測(cè)血清、血漿樣本中的OxLDL含量。OxLDL（氧化低密度脂蛋白）是一種被氧化修飾的低密度脂蛋白。它與動(dòng)脈粥樣硬化等疾病密切相關(guān)。，OxLDL可被巨噬細(xì)胞攝取形成泡沫細(xì)胞，促進(jìn)脂質(zhì)斑塊形成，還能引發(fā)炎癥反應(yīng)、影響血管內(nèi)皮功能，進(jìn)而參與疾病的發(fā)生發(fā)展。試劑盒檢測(cè)范圍為0.39 nmol/mL-100 nmol/mL，可為心血管疾病機(jī)制研究及氧化應(yīng)激相關(guān)模型評(píng)估提供關(guān)鍵數(shù)據(jù)；適用于基礎(chǔ)科研中探究豬模型動(dòng)脈粥樣硬化病理進(jìn)程、評(píng)估抗氧化干預(yù)效果或代謝性疾病相關(guān)研究，為心血管及代謝領(lǐng)域研究者提供穩(wěn)定可靠的氧化應(yīng)激生物標(biāo)志物檢測(cè)方案。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

OxLDL
種屬：

Sus scrofa (Pig)
樣本類型：

serum, plasma
檢測(cè)范圍：

0.39 nmol/mL-100 nmol/mL
靈敏度：

0.39 nmol/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長：

450 nm
研究領(lǐng)域：

Others
測(cè)定原理：

quantitative
測(cè)定方法：

Competitive

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig OxLDL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:200	Average %	97
	Range %	92-105
1:400	Average %	95
	Range %	83-100
1:800	Average %	95
	Range %	89-100
1:1600	Average %	89
	Range %	85-99

回收率：

The recovery of pig OxLDL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-100
EDTA plasma (n=4)	94	87-98

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

nmol/ml	OD1	OD2	Average
100	0.349	0.342	0.346
25	0.627	0.638	0.633
6.25	0.925	0.914	0.92
1.56	1.136	1.153	1.145
0.39	1.594	1.625	1.61
0	1.899	1.864	1.882

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

引用文獻(xiàn)

Antioxidative Effects of Olive Polyphenols Compared to Vitamin E in Piglets Fed a Diet Rich in N-3 PUFA Jakob Leskovec, et al,Animals,2019

Pig oxidized low density lipoprotein,OxLDL ELISA Kit

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