Pig interleukin 4,IL-4 ELISA Kit

Pig interleukin 4,IL-4 ELISA Kit

產(chǎn)品詳情

引用文獻(xiàn)

產(chǎn)品評價(jià)

靶點(diǎn)詳情

產(chǎn)品詳情

引用文獻(xiàn)

產(chǎn)品評價(jià)

相關(guān)產(chǎn)品

靶點(diǎn)詳情

Most popular with customers

產(chǎn)品中心

信號通路

技術(shù)信息

聯(lián)系信息

文獻(xiàn)引用 (5) 說明書下載

中文名稱：

豬白介素4(IL-4)酶聯(lián)免疫試劑盒
貨號：

CSB-E06785p
規(guī)格：

96T/48T
價(jià)格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

產(chǎn)品詳情
產(chǎn)品評價(jià)
引用文獻(xiàn)
相關(guān)產(chǎn)品
靶點(diǎn)詳情

產(chǎn)品描述：

豬白介素4(IL-4)酶聯(lián)免疫試劑盒（CSB-E06785p）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的IL4含量。IL4是重要的靶點(diǎn)。它是白細(xì)胞介素家族成員，參與免疫調(diào)節(jié)、炎癥反應(yīng)等多種生理病理過程。研究機(jī)制上，IL4與其受體結(jié)合后，激活細(xì)胞內(nèi)信號通路，影響Th2細(xì)胞分化等。對其研究有助于理解相關(guān)疾病發(fā)病機(jī)制及開發(fā)治療藥物。試劑盒檢測范圍為6.25 pg/mL-400 pg/mL，該產(chǎn)品可廣泛應(yīng)用于豬免疫相關(guān)疾病模型構(gòu)建、疫苗效力評估、炎癥機(jī)制研究等科研領(lǐng)域，為探索IL - 4在感染性疾病、自身免疫性疾病及過敏性反應(yīng)中的作用提供可靠工具，尤其適用于比較醫(yī)學(xué)研究和畜牧獸醫(yī)領(lǐng)域的免疫學(xué)實(shí)驗(yàn)需求。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

IL4Interleukin-4 ELISA Kit; IL-4 ELISA Kit; B-cell stimulatory factor 1 ELISA Kit; BSF-1 ELISA Kit; Lymphocyte stimulatory factor 1 ELISA Kit
縮寫：

IL4
Uniprot No.：

Q04745
種屬：

Sus scrofa (Pig)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates
檢測范圍：

6.25 pg/mL-400 pg/mL
靈敏度：

1.56 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig IL-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	86
	Range %	82-91
1:2	Average %	90
	Range %	85-95
1:4	Average %	89
	Range %	84-93
1:8	Average %	96
	Range %	92-101

回收率：

The recovery of pig IL-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	100	95-105
EDTA plasma (n=4)	92	86-96

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
400	2.719	2.652	2.686	2.492
200	1.962	1.861	1.912	1.718
100	1.213	1.204	1.209	1.015
50	0.748	0.689	0.719	0.525
25	0.551	0.527	0.539	0.345
12.5	0.355	0.374	0.365	0.171
6.25	0.295	0.272	0.284	0.090
0	0.192	0.196	0.194

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

Effects of Magnolia officinalis extract on the growth performance and immune function of weaned piglets C Zhang, B Liu, Z Cui, K Wu, H Huang, Y Wang,Porcine Health Management,2025
Development and immunogenicity evaluation of a quadruple-gene-deleted pseudorabies virus strain H Li, R Zhang, J Qu, Y Kang, J Zhang, R Guo,Frontiers in Microbiology,2024
Streptococcus suis Serotype 2 Infection Causes Host Immunomodulation Through Induction of Thymic Atrophy Wang S,Infect Immun,2020
Oral administration of Lactobacillus delbrueckii during the suckling period improves intestinal integrity after weaning in piglets Yinghui Li, et al,Journal of Functional Foods,2019
Effects of Selective Inhibition of PDE4 by YM976 on Airway Reactivity and Cough in Ovalbumin-Sensitized Guinea Pigs Mokr J.et al,Adv Exp Med Biol.,2016

功能：

Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Positively regulates IL31RA expression in macrophages. Stimulates autophagy in dendritic cells by interfering with mTORC1 signaling and through the induction of RUFY4.
基因功能參考文獻(xiàn)：
1. IL-4- and IL-10-producing invariant NKT cells inhibit the Th1 cell response, but not the Th17 cell response PMID: 21572032
2. IL-4 induced activation of Akt/SREBP-1/lipid biosynthesis in EC, resulting in protection against membrane attack complex and melittin, in association with mitochondrial protection. PMID: 20017192
3. There was no significant difference of IL-4 production between fresh and frozen peripheral blood mononuclear cells. PMID: 19766617
4. The endothelium and subendothelium of porcine iliac arteries that are transduced with recombinant pig IL-4 are effectively protected from complement-dependent immediate injury after perfusion with human blood. PMID: 17082655
5. unlike in humans and mice, porcine IL-4 blocks antibody and IL-6 secretion and suppresses antigen-stimulated proliferation of B cells PMID: 18761033
顯示更多

收起更多
亞細(xì)胞定位：

Secreted.
蛋白家族：

IL-4/IL-13 family
數(shù)據(jù)庫鏈接：

KEGG: ssc:397225

STRING: 9823.ENSSSCP00000015193

UniGene: Ssc.15837

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.