Pig glucagon like peptide 2(GLP2) ELISA kit

產(chǎn)品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

豬胰高血糖素樣肽2(GLP2)酶聯(lián)免疫試劑盒（CSB-EQ027317PI）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的GLP2含量。GLP-2（胰高血糖素樣肽-2）是一種由腸道L細(xì)胞合成的單鏈多肽類激素，主要存在于胃腸道中，具有促進(jìn)營養(yǎng)吸收和增強(qiáng)腸道屏障功能的作用。其研究機(jī)制涉及調(diào)節(jié)腸道黏膜免疫，可能通過促進(jìn)T、B淋巴細(xì)胞增殖、抑制凋亡以及調(diào)節(jié)精氨酸、瓜氨酸和谷氨酰胺的代謝水平來增強(qiáng)免疫功能。試劑盒檢測范圍為2.4 pg/mL-150 pg/mL，本產(chǎn)品適用于動物模型研究、胃腸功能機(jī)制探索、營養(yǎng)代謝調(diào)控等科研場景，為腸道健康、病理生理學(xué)及藥效評估等基礎(chǔ)研究提供可靠工具本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

GLP2
種屬：

Sus scrofa (Pig)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

2.4 pg/mL-150 pg/mL
靈敏度：

0.6 pg/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Metabolism
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig GLP2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5	Average %	102
	Range %	95-109
1:10	Average %	95
	Range %	91-99
1:20	Average %	88
	Range %	83-95
1:40	Average %	99
	Range %	96-106

回收率：

The recovery of pig GLP2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	104	100-108
EDTA plasma (n=4)	93	89-97

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.