Pig coagulation factor VIII(FVIII) ELISA Kit

Pig coagulation factor VIII(FVIII) ELISA Kit

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說明書下載

中文名稱：

豬凝血因子VIII(FVIII)酶聯免疫試劑盒
貨號：

CSB-E15000p
規格：

96T/48T
價格：

￥4200/￥3000
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產品描述：

豬凝血因子VIII(FVIII)酶聯免疫試劑盒（CSB-E15000p）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的F8含量。試劑盒檢測范圍為62.5 ng/mL-4000 ng/mL，適用于出血性疾病機制研究、凝血調節藥物開發、動物模型凝血功能評估等科研領域，特別適用于畜牧科學、獸醫學研究及生物制藥領域的凝血相關實驗分析。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

F8 ELISA kit; CF8Coagulation factor VIII ELISA kit; Procoagulant component ELISA kit
縮寫：

F8
Uniprot No.：

P12263
種屬：

Sus scrofa (Pig)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

62.5 ng/mL-4000 ng/mL
靈敏度：

15.62 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig FVIII in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:100	Average %	89
	Range %	84-95
1:200	Average %	94
	Range %	89-99
1:400	Average %	93
	Range %	86-100
1:800	Average %	98
	Range %	91-103

回收率：

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml

OD1

OD2

Average

4000

0.134

0.128

0.131

2000

0.221

0.237

0.229

1000

0.384

0.398

0.391

500

0.615

0.629

0.622

250

0.868

0.881

0.875

125

1.214

1.253

1.234

62.5

1.649

1.684

1.667

2.236

2.125

2.181

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

功能：

Factor VIII, along with calcium and phospholipid, acts as a cofactor for factor IXa when it converts factor X to the activated form, factor Xa.
基因功能參考文獻：
1. It was concluded that VEGF and factor VIII are important growth factors associated with fetal development in pigs and are identified in all uterine segments. PMID: 28237345
2. Report Factor VIII organisation on nanodiscs with different lipid composition. PMID: 25589466
3. thrombin stimulates transglutaminase activity in articular cartilage by directly cleaving factor XIII and by receptor-mediated up-regulation of factor XIII synthesis PMID: 14983233
4. cupredoxin-like A1 subdomains in fVIII contain inter-species differences that are a result of selective pressure on the dissociation rate constant PMID: 16513639
亞細胞定位：

Secreted, extracellular space.
蛋白家族：

Multicopper oxidase family
數據庫鏈接：

KEGG: ssc:397339

STRING: 9823.ENSSSCP00000028556

UniGene: Ssc.16043

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.