Pig Trypsin ELISA Kit

說明書下載

中文名稱：

豬胰蛋白酶(Trypsin)酶聯免疫試劑盒
貨號：

CSB-E15767p
規格：

96T/48T
價格：

￥4200/￥3000
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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豬胰蛋白酶(Trypsin)酶聯免疫試劑盒（CSB-E15767p）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿樣本中的Trypsin含量。Trypsin即胰蛋白酶，是一種絲氨酸蛋白酶，廣泛存在于脊椎動物消化系統中。其能特異性切割精氨酸和賴氨酸殘基羧基端肽鍵。在研究機制上，常作為工具酶用于蛋白質分析，通過切割蛋白質來確定其結構和功能。試劑盒檢測范圍為3.12 ng/mL-200 ng/mL，可用于動物模型研究、胰腺外分泌功能評估、飼料營養代謝分析或酶活性相關機制探索等科研場景，為消化生理學、病理學及生物醫藥開發提供可靠的數據支持。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

Trypsin
種屬：

Sus scrofa (Pig)
樣本類型：

serum, plasma
檢測范圍：

3.12 ng/mL-200 ng/mL
靈敏度：

0.78 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig trypsin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

Sample

Serum(n=4)

1:5

Average %

Range %

83-97

1:10

Average %

Range %

80-90

1:20

Average %

Range %

84-99

1:40

Average %

Range %

88-100

回收率：

The recovery of pig trypsin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	93-103
EDTA plasma (n=4)	99	94-105

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
200	2.192	2.131	2.162	2.029
100	1.769	1.834	1.802	1.669
50	1.289	1.319	1.304	1.171
25	0.824	0.867	0.846	0.713
12.5	0.543	0.577	0.560	0.427
6.25	0.372	0.337	0.355	0.222
3.12	0.288	0.259	0.274	0.141
0	0.137	0.129	0.133

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

Pig Trypsin ELISA Kit

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