Pig Keratinocyte Growth Factor,KGF ELISA Kit

Pig Keratinocyte Growth Factor,KGF ELISA Kit

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中文名稱：

豬角化細胞生長因子(KGF)酶聯(lián)免疫試劑盒
貨號：

CSB-E06800p
規(guī)格：

96T/48T
價格：

￥4200/￥3000
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產(chǎn)品描述：

豬角化細胞生長因子(KGF)酶聯(lián)免疫試劑盒（CSB-E06800p）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿樣本中的FGF7含量。FGF7 即成纖維細胞生長因子 7，對上皮細胞的增殖、分化和遷移起重要作用。其研究機制主要聚焦于與特異性受體結(jié)合，激活下游信號通路，影響細胞生理過程。在組織修復(fù)、腫瘤發(fā)生發(fā)展等方面有潛在研究價值。試劑盒檢測范圍為3.12 pg/mL-200pg/mL，適用于豬模型中與表皮損傷修復(fù)、炎癥反應(yīng)或毛發(fā)再生相關(guān)的分子機制研究。可廣泛應(yīng)用于農(nóng)業(yè)科研中豬皮膚疾病模型構(gòu)建、藥物干預(yù)效果評估或生物工程材料促愈活性分析等基礎(chǔ)研究場景，為探索KGF在豬生理病理過程中的作用提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

FGF7Fibroblast growth factor 7 ELISA kit; FGF-7 ELISA kit; Heparin-binding growth factor 7 ELISA kit; HBGF-7 ELISA kit; Keratinocyte growth factor ELISA kit; KGF ELISA kit
縮寫：

FGF7
Uniprot No.：

Q9N198
種屬：

Sus scrofa (Pig)
樣本類型：

serum, plasma
檢測范圍：

3.12 pg/mL-200pg/mL
靈敏度：

0.78 pg/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig KGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	97
	Range %	92-101
1:2	Average %	88
	Range %	84-92
1:4	Average %	90
	Range %	85-98
1:8	Average %	106
	Range %	101-114

回收率：

The recovery of pig KGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-100
EDTA plasma (n=4)	84	80-88

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
200	1.815	1.731	1.773	1.632
100	1.128	1.173	1.151	1.010
50	0.789	0.827	0.808	0.667
25	0.503	0.526	0.515	0.374
12.5	0.312	0.309	0.311	0.170
6.25	0.251	0.271	0.261	0.120
3.12	0.188	0.178	0.183	0.042
0	0.144	0.138	0.141

數(shù)據(jù)處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

Characterization of bladder acellular matrix hydrogel with inherent bioactive factors Dan Jiang.et al,Materials Science and Engineering: C,2017

功能：

Plays an important role in the regulation of embryonic development, cell proliferation and cell differentiation. Required for normal branching morphogenesis. Growth factor active on keratinocytes. Possible major paracrine effector of normal epithelial cell proliferation.
亞細胞定位：

Secreted.
蛋白家族：

Heparin-binding growth factors family
數(shù)據(jù)庫鏈接：

STRING: 9823.ENSSSCP00000025433

UniGene: Ssc.15923

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.