Pig Interleukin 1β,IL-1β ELISA Kit

Pig Interleukin 1β,IL-1β ELISA Kit

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文獻引用 (29) 評價 (1) 說明書下載

中文名稱：

豬白介素1β (IL-1β)酶聯(lián)免疫試劑盒
貨號：

CSB-E06782p
規(guī)格：

96T/48T
價格：

￥3800/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產(chǎn)品描述：

豬白介素1β (IL-1β)酶聯(lián)免疫試劑盒（CSB-E06782p）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的IL1B含量。IL1B，即白細胞介素-1β，是一種強效的促炎細胞因子，主要由單核細胞、巨噬細胞和樹突狀細胞產(chǎn)生。它在炎癥和免疫反應(yīng)中起關(guān)鍵作用，通過炎癥小體激活和蛋白水解過程產(chǎn)生活性形式，進而調(diào)節(jié)多種細胞活動，包括細胞增殖、分化和凋亡。試劑盒檢測范圍為3.9 pg/mL-250 pg/mL，該產(chǎn)品可廣泛應(yīng)用于動物醫(yī)學(xué)研究領(lǐng)域，包括但不限于感染性疾病模型的炎癥反應(yīng)評估、免疫調(diào)節(jié)藥物開發(fā)中的療效監(jiān)測，以及疫苗研發(fā)過程中細胞因子動態(tài)變化分析，為科研人員提供穩(wěn)定可靠的檢測平臺。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

IL1BInterleukin-1 beta ELISA kit; IL-1 beta ELISA kit
縮寫：

IL1B
Uniprot No.：

P26889
種屬：

Sus scrofa (Pig)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates
檢測范圍：

3.9 pg/mL-250 pg/mL
靈敏度：

0.975 pg/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig IL-1β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	88
	Range %	80-100
1:2	Average %	96
	Range %	91-103
1:4	Average %	95
	Range %	90-110
1:8	Average %	95
	Range %	85-105

回收率：

The recovery of pig IL-1β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	83-104
EDTA plasma (n=4)	98	90-108

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
250	2.675	2.815	2.745	2.597
125	2.154	2.147	2.151	2.003
62.5	1.312	1.335	1.324	1.176
31.25	0.788	0.764	0.776	0.628
15.6	0.377	0.356	0.367	0.219
7.8	0.264	0.269	0.267	0.119
3.9	0.190	0.188	0.189	0.041
0	0.153	0.143	0.148

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

Effects of Magnolia officinalis extract on the growth performance and immune function of weaned piglets C Zhang, B Liu, Z Cui, K Wu, H Huang, Y Wang,Porcine Health Management,2025
Hydroxy-Selenomethionine Supplementation During Gestation and Lactation Improve Reproduction of Sows by Enhancing the Antioxidant Capacity and Immunity Under Heat Stress Conditions J Wang, H Sun, Z Peng, SQ Wang, YQ Yan, WC Luo,Antioxidants,2025
Taurine ameliorates deoxynivalenol-induced intestinal injury in piglets: Restoration of mitochondrial function linked to the PGC1α-NRF1/2 axis X Ji, H Ding, F Zhou, F Zhang, D Wu,Ecotoxicology and Environmental Safety,2025
Nervonic acid triggered ovarian inflammation by inducing mitochondrial oxidative stress to activate NLRP3/IL-1β pathway X Zeng, X Fan, H Yu, S Cai, L Zhou, H Wu,Journal of Advanced Research,2024
Disruption of pulmonary microvascular endothelial barrier by dysregulated claudin-8 and claudin-4: uncovered mechanisms in porcine reproductive and respiratory syndrome virus infection W Sun,Cellular and molecular life sciences,2024
β-hydroxybutyrate administration improves liver injury and metabolic abnormality in postnatal growth retardation piglets C Wang,Frontiers in veterinary science,2023
Effects of Gamma-Ray Irradiation of Bacteria Colonies in Animal Feeds and on Growth and Gut Health of Weaning Piglets H Wei,Animals,2023
Mannan oligosaccharides selenium ameliorates intestinal mucosal barrier, and regulate intestinal microbiota to prevent Enterotoxigenic Escherichia coli -induced diarrhea in weaned piglets A Zha,Ecotoxicology and environmental safety,2023
Interaction between Porcine Alveolar Macrophage-Tang Cells and Streptococcus suis Strains of Different Virulence: Phagocytosis and Apoptosis S Li,Microorganisms,2023
Multi-omics unravel the compromised mucosal barrier function linked to aberrant mucin O-glycans in a pig model B Xia,International journal of biological macromolecules,2023
Effects of chlortetracycline on growth performance and intestinal functions in weaned piglets H Han,Journal of applied microbiology,2023
Recombinant characteristics, pathogenicity, and viral shedding of a novel PRRSV variant derived from twice inter-lineage recombination XY Cui,Veterinary microbiology,2023
Gastrointestinal Nutrients-Microbiome-Host Interactions in Livestock H Luo,/,2024
Dietary Litsea cubeba essential oil supplementation improves growth performance and intestinal health of weaned piglets Z Yang,Animal Nutrition,2022
Mucin O-glycan-microbiota axis orchestrates gut homeostasis in a diarrheal pig model B Xia,Microbiome,2022
Intrauterine Growth Retardation Affects Intestinal Health of Suckling Piglets via Altering Intestinal Antioxidant Capacity, Glucose Uptake, Tight Junction, and Immune Responses X Tang,Oxidative Medicine and Cellular Longevity,2022
Translational Approach to Induce and Evaluate Verocytotoxic E. coli O138 Based Disease in Piglets L Ross,animals,2021
Dietary resveratrol attenuation of intestinal inflammation and oxidative damage is linked to the alteration of gut microbiota and butyrate in piglets challenged with deoxynivalenol Y Qiu,Journal of Animal Science and Biotechnology,2021
MiR-339 attenuates LPS-induced intestinal epithelial cells inflammatory responses and apoptosis by targeting TLR4 M Xie,Genes & Genomics,2020
Oral administration of Lactobacillus delbrueckii during the suckling period improves intestinal integrity after weaning in piglets Yinghui Li, et al,Journal of Functional Foods,2019
Mycoplasma hyopneumoniae Mhp597 is a Cytotoxicity, Inflammation and Immunosuppression Associated Nuclease PengLi,Veterinary Microbiology,2019
Dietary xylo-oligosaccharide improves intestinal function in weaned piglets Jie Yin, et al,Food & Function,2019
Probiotics-fermented Massa Medicata Fermentata ameliorates weaning stress in piglets related to improving intestinal homeostasis Yanbo Wang, et al,Applied Microbiology and Biotechnology,2018
Dietary resveratrol improves antioxidant status of sows and piglets and regulates antioxidant gene expression in placenta by Keap1-Nrf2 pathway and Sirt1 Qingwei Meng.et al,J Anim Sci Biotechnol.,2018
Influences of Copper/Zinc-Loaded Montmorillonite on Growth Performance, Mineral Retention, Intestinal Morphology, Mucosa Antioxidant Capacity Jiao LF.et al,Biol Trace Elem Res,2018
Olive oil bioactives protect pigs against experimentally-induced chronic inflammation independently of alterations in gut microbiota Martin Liehr.et al,PLOS ONE,2017
Oyster crude polysaccharides attenuates lipopolysaccharide-induced cytokines production and PPARγ expression in weanling piglets. Yin G.et al,Springerplus.,2016
Effect of low dosage of chito-oligosaccharide supplementation on intestinal morphology, immune response, antioxidant capacity, and barrier function in weaned piglets Xiong X.et al,J Anim Sci.,2015
Effects of the antimicrobial peptide cecropin AD on performance and intestinal health in weaned piglets challenged with Escherichia coli. Wu S et al,Peptides,2012

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平均分:

5.0分 - 1 個評價

樣品類型：細胞培養(yǎng)上清液

樣品信息：其他

稀釋比：沒有稀釋

產(chǎn)品評價：結(jié)果很滿意，也是對比了別家的試劑盒，最后發(fā)現(xiàn)還是華美的好用，我用的是細胞培養(yǎng)基上清，別家的試劑盒有干擾因素，華美的沒有，試了好幾家，也就這個牌子的好用。

By 高老師

功能：

Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells. Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6.
基因功能參考文獻：
1. Analysis of host-protein interactions for African swine fever virus L83L identified IL-1beta as its host ligand. A recombinant African swine fever virus lacking the L83L was developed from the highly virulent field isolate Georgia2007 and was used to show that L83L is a non-essential gene. PMID: 29605728
2. This study showed that the presence of embryos in oviducts on days 2-3 after mating may influence the oviductal expression of the members of the IL-1beta system, determining the action of IL-1beta, which may be considered to be the earliest sign of pregnancy in pigs. PMID: 28532837
3. This study showed that classical swine fever virus and p7 protein induced IL-1beta secretion and that p7 protein was a short-lived protein degraded by the proteasome. PMID: 25146005
4. local expression of IL-1beta and IL-8 in non-bacterial thrombotic endocarditis, Staphylococcus aureus infective endocarditis, animals with S. aureus sepsis without endocarditis and controls PMID: 23879680
5. IL1B regulates expression of IL1R1 and IL1RAP and stimulates expression of PTGS1 and PTGS2 that are considered to be the most rate-limiting enzymes for endometrial synthesis of prostaglandins during the peri-implantation period of pregnancy in pigs. PMID: 22572995
6. the results presented here strongly suggest IL-1beta as a key molecule guiding tissue remodelling events after myocardial infarction. PMID: 20678474
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亞細胞定位：

Cytoplasm, cytosol. Lysosome. Secreted, extracellular exosome. Secreted.
蛋白家族：

IL-1 family
數(shù)據(jù)庫鏈接：

KEGG: ssc:397122

STRING: 9823.ENSSSCP00000008636

UniGene: Ssc.28829

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.