Pig Interleukin 18,IL-18 ELISA Kit
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中文名稱：豬白介素18(IL-18)酶聯(lián)免疫試劑盒
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貨號：CSB-E06781p
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規(guī)格：96T/48T
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價格：￥3600/￥2500
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其他：
產(chǎn)品詳情
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產(chǎn)品描述：豬白介素18(IL-18)酶聯(lián)免疫試劑盒（CSB-E06781p）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的IL18含量。IL18 即白細(xì)胞介素 18，是一種多功能促炎細(xì)胞因子，在先天免疫和獲得性免疫中均發(fā)揮重要作用。其研究機制主要圍繞它能誘導(dǎo) T 細(xì)胞和自然殺傷細(xì)胞產(chǎn)生γ-干擾素，增強自然殺傷細(xì)胞的細(xì)胞毒活性，在炎癥、免疫相關(guān)疾病研究中備受關(guān)注。試劑盒檢測范圍為31.25 pg/mL-2000 pg/mL，適用于科研場景中動態(tài)監(jiān)測實驗動物模型（如細(xì)菌/病毒感染、免疫調(diào)節(jié)研究）的免疫應(yīng)答水平；為獸醫(yī)學(xué)、比較免疫學(xué)及動物疾病模型研究提供可靠工具，助力科研人員解析IL - 18在豬免疫系統(tǒng)中的分子機制與調(diào)控網(wǎng)絡(luò)。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
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別名：IL18 ELISA Kit; IGIF ELISA Kit; Interleukin-18 ELISA Kit; IL-18 ELISA Kit; Interferon gamma-inducing factor ELISA Kit; IFN-gamma-inducing factor ELISA Kit; Interleukin-1 gamma ELISA Kit; IL-1 gamma ELISA Kit
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縮寫：
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Uniprot No.：
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種屬：Sus scrofa (Pig)
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樣本類型：serum, plasma, tissue homogenates
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檢測范圍：31.25 pg/mL-2000 pg/mL
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靈敏度：7.81 pg/mL
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反應(yīng)時間：1-5h
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樣本體積：50-100ul
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檢測波長：450 nm
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研究領(lǐng)域：Immunology
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測定原理：quantitative
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測定方法：Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度：
To assess the linearity of the assay, samples were spiked with high concentrations of pig IL-18 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 88 Range % 82-92 1:2 Average % 100 Range % 95-105 1:4 Average % 91 Range % 85-97 1:8 Average % 97 Range % 91-103 -
回收率：
The recovery of pig IL-18 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 89 84-93 EDTA plasma (n=4) 94 91-98 -
標(biāo)準(zhǔn)曲線：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
pg/ml OD1 OD2 Average Corrected 2000 2.562 2.687 2.625 2.443 1000 1.839 1.865 1.852 1.670 500 1.104 1.135 1.120 0.938 250 0.643 0.638 0.641 0.459 125 0.412 0.435 0.424 0.242 62.5 0.321 0.341 0.331 0.149 31.25 0.262 0.248 0.255 0.073 0 0.181 0.183 0.182 -
數(shù)據(jù)處理：
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貨期：3-5 working days
引用文獻(xiàn)
- Interaction between Porcine Alveolar Macrophage-Tang Cells and Streptococcus suis Strains of Different Virulence: Phagocytosis and Apoptosis S Li,Microorganisms,2023
- Multi-omics unravel the compromised mucosal barrier function linked to aberrant mucin O-glycans in a pig model B Xia,International journal of biological macromolecules,2023
- Effects of resveratrol and its derivative pterostilbene on hepatic injury and immunological stress of weaned piglets challenged with lipopolysaccharide Y Li,Journal of Animal Science,2022
- Mucin O-glycan-microbiota axis orchestrates gut homeostasis in a diarrheal pig model B Xia,Microbiome,2022
產(chǎn)品評價
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靶點詳情
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功能：A proinflammatory cytokine primarily involved in polarized T-helper 1 (Th1) cell and natural killer (NK) cell immune responses. Upon binding to IL18R1 and IL18RAP, forms a signaling ternary complex which activates NF-kappa-B, triggering synthesis of inflammatory mediators. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells and natural killer (NK) cells.
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基因功能參考文獻(xiàn)：
- Study reports that the monocyte-derived cytokines IL-12 and IL-18 act synergistically on porcine gammadelta T cells to induce IFN-gamma production. Additional stimuli such as the mitogen ConA and IL-2 are necessary to activate the cells for enhanced proliferation. PMID: 25036760
- IL-18 concentration in saliva was significantly increased during a 60-min acute immobilization stress in thirteen 5-month-old pigs. These results are the first evidence of a stress-related change of IL-18 in pig saliva. PMID: 21682650
- endometrial expression of CASP1 and IL18 associated with pregnancy establishment; alteration of CASP1 and IL18 following premature exposure of uterus to estrogen during early pregnancy may contribute to conceptus loss between Days 15 to 18 of pregnancy PMID: 20380728
- Porcine IL-18 regulates anti-pig cellular rejection in C57BL/6 mice. PMID: 17079874
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亞細(xì)胞定位：Cytoplasm. Secreted.
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蛋白家族：IL-1 family
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數(shù)據(jù)庫鏈接：
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