Pig Complement 3,C3 ELISA Kit

Pig Complement 3,C3 ELISA Kit

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中文名稱：

豬補體蛋白3(C3)酶聯免疫試劑盒
貨號：

CSB-E06920p
規格：

96T/48T
價格：

￥4200/￥3000
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產品描述：

豬補體蛋白3(C3)酶聯免疫試劑盒（CSB-E06920p）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的C3含量。C3作為靶點頗具研究價值。它是補體系統的關鍵成分，在免疫防御等多種生理過程中發揮核心作用。其研究機制主要聚焦于補體激活途徑及下游效應，旨在通過調控C3功能，開發針對自身免疫病、炎癥等疾病的治療新策略。試劑盒檢測范圍為0.625 μg/mL-10 μg/mL，在科研領域，本產品可用于探究豬免疫相關疾病（如細菌/病毒感染、自身免疫性疾病）模型中補體系統的激活機制，評估疫苗或藥物干預對補體通路的影響，亦可應用于比較不同遺傳背景或飼養條件下豬群體C3表達水平的差異。組織勻漿檢測功能還可支持局部炎癥或器官特異性免疫反應的研究，例如腸道、肝臟等組織中補體活化與病理進程的關聯分析。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

C3 ELISA Kit; Complement C3 [Cleaved into: Complement C3 beta chain; C3-beta-c ELISA Kit; C3bc); Complement C3 alpha chain; C3a anaphylatoxin; Acylation stimulating protein ELISA Kit; ASP ELISA Kit; C3adesArg); Complement C3b alpha' chain; Complement C3c alpha' chain fragment 1; Complement C3dg fragment; Complement C3g fragment; Complement C3d fragment; Complement C3f fragment; Complement C3c alpha' chain fragment 2] ELISA Kit
縮寫：

C3
Uniprot No.：

P01025
種屬：

Sus scrofa (Pig)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.625 μg/mL-10 μg/mL
靈敏度：

0.625 μg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig C3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	96
	Range %	91-100
1:200	Average %	93
	Range %	84-103
1:400	Average %	95
	Range %	91-101
1:800	Average %	93
	Range %	83-102

回收率：

The recovery of pig C3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	88-108
EDTA plasma (n=4)	95	90-100

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

μg/ml	OD1	OD2	Average
10	0.083	0.084	0.084
5	0.185	0.176	0.181
2.5	0.349	0.352	0.351
1.25	0.769	0.776	0.773
0.625	1.285	1.292	1.289
0	2.140	2.151	2.146

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

Nonspecific protection of heat-inactivated Mycobacterium bovis against Salmonella Choleraesuis infection in pigs R Vaz,Veterinary research,2023
Randomized comparison of sevoflurane versus propofol on organ blood flow after partial thoracic aortic cross-clamping Paloma Morillas-Sendin, et al,International Journal Of Clinical And Experimental Medicine,2019
Effects of Clostridium butyricum or in combination with Bacillus licheniformis on the growth performance, blood indexes, and intestinal barrier function of weanling piglets Zong X, et al,Livestock Science,2018
Effect of dietary copper source (inorganic vs. chelated) on immune response, mineral status, and fecal mineral excretion in nursery piglets Peng Liao.et al,Food and Agricultural Immunology ,2017

功能：

C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.; Derived from proteolytic degradation of complement C3, C3a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes. In chronic inflammation, acts as a chemoattractant for neutrophils.; Acts as a chemoattractant for neutrophils in chronic inflammation.; adipogenic hormone that stimulates triglyceride (TG) synthesis and glucose transport in adipocytes, regulating fat storage and playing a role in post-prandial TG clearance. Appears to stimulate TG synthesis via activation of the PLC, MAPK and AKT signaling pathways. Ligand for C5AR2. Promotes the phosphorylation, ARRB2-internalization and recycling of C5AR2.
亞細胞定位：

Secreted.
數據庫鏈接：

KEGG: ssc:397072

STRING: 9823.ENSSSCP00000014399

UniGene: Ssc.61

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.