Pig Brain-derived neurotrophic factor(BDNF) ELISA kit

說明書下載

中文名稱：

豬腦源性神經營養因子(BDNF)酶聯免疫試劑盒
貨號：

CSB-EL002655PI
規格：

96T/48T
價格：

￥4200/￥3000
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產品描述：

豬腦源性神經營養因子(BDNF)酶聯免疫試劑盒（CSB-EL002655PI）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的BDNF含量。BDNF即腦源性神經營養因子，在神經系統發育和維持中扮演關鍵角色。研究顯示，它能促進神經元存活、生長和分化，增強突觸可塑性。其機制是與特定受體結合，激活細胞內信號通路，進而調控基因表達，影響神經系統功能。試劑盒檢測范圍為9.375 ng/mL-600 ng/mL，豬模型研究中神經退行性疾病機制探索、藥物干預效果評估或應激狀態下神經營養因子動態變化分析等科研場景，以及神經科學、畜牧獸醫及比較醫學領域的實驗研究本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

BDNFBrain-derived neurotrophic factor ELISA kit; BDNF) [Cleaved into: BDNF precursor form ELISA kit; ProBDNF)] ELISA kit
縮寫：

BDNF
Uniprot No.：

P14082
種屬：

Sus scrofa (Pig)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

9.375 ng/mL-600 ng/mL
靈敏度：

2.35 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cardiovascular
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig BDNF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	96
	Range %	91-102
1:2	Average %	102
	Range %	97-107
1:4	Average %	109
	Range %	105-113
1:8	Average %	103
	Range %	96-107

回收率：

The recovery of pig BDNF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	102	98-105
EDTA plasma (n=4)	86	93-92

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
600	2.134	2.081	2.108	2.016
300	1.537	1.623	1.580	1.488
150	1.167	1.123	1.145	1.053
75	0.769	0.774	0.772	0.680
37.5	0.513	0.503	0.508	0.416
18.75	0.353	0.344	0.349	0.257
9.375	0.227	0.221	0.224	0.132
0	0.091	0.093	0.092

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

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功能：

Important signaling molecule that activates signaling cascades downstream of NTRK2. During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability.; Important signaling molecule that activates signaling cascades downstream of NTRK2. Activates signaling cascades via the heterodimeric receptor formed by NGFR and SORCS2. Signaling via NGFR and SORCS2 plays a role in synaptic plasticity and long-term depression (LTD). Binding to NGFR and SORCS2 promotes neuronal apoptosis. Promotes neuronal growth cone collapse.
基因功能參考文獻：
1. measurement of brain derived neurotrophic factor in serum brings a practical approach to study the effects of environmental enrichment on neurobiological changes in domestic animals PMID: 30007130
2. Lf upregulated several canonical signaling pathways associated with neurodevelopment and cognition and influenced ~10 genes involved in the brain-derived neurotrophin factor (BDNF) signaling pathway. PMID: 25146846
3. Taken together, these data indicate that recurrent tethering stress in sows over 4.5 years results in a loss of neurotrophic support by BDNF, mediated by an overactive neuroendocrine system. PMID: 22584108
4. FiO2 used for resuscitation affects matrix metalloproteinases MMP-9 and MMP-2, caspase-3 and BDNF PMID: 21151608
亞細胞定位：

Secreted.; [BDNF precursor form]: Secreted.
蛋白家族：

NGF-beta family
組織特異性：

Brain and central nervous system.
數據庫鏈接：

KEGG: ssc:397495

STRING: 9823.ENSSSCP00000014166

UniGene: Ssc.16243

Pig Brain-derived neurotrophic factor(BDNF) ELISA kit

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