Mouse terminal complement complex C5b-9,TCC C5b-9 ELISA Kit

產品詳情

文獻引用 (4) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

小鼠末端補體復合物C5b-9(TCC C5b-9)酶聯免疫試劑盒（CSB-E08710m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養上清液、組織勻漿樣本中的TCC C5b9含量。小鼠末端補體復合物C5b-9（TCC C5b-9）是補體系統激活終末階段的產物，通過介導細胞膜穿孔參與免疫防御和炎癥反應調控。其在膜性腎病等免疫相關疾病中發揮重要作用，研究其機制有助于揭示疾病發病機制，為疾病治療提供新靶點。試劑盒檢測范圍為0.78 ng/mL-50 ng/mL，該產品適用于科研場景中炎癥性疾病模型評估、補體通路功能研究或藥物干預效果分析，為探索免疫調節機制提供可靠工具，所有應用僅限于實驗室研究。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

TCC C5b9
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates
檢測范圍：

0.78 ng/mL-50 ng/mL
靈敏度：

0.195 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Others
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse TCC C5b-9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	88
	Range %	84-96
1:200	Average %	98
	Range %	91-105
1:400	Average %	95
	Range %	91-99
1:800	Average %	92
	Range %	88-96

回收率：

The recovery of mouse TCC C5b-9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-97
EDTA plasma (n=4)	97	92-105

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.