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中文名稱：小鼠分泌型免疫球蛋白A(sIgA)酶聯免疫試劑盒
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貨號：CSB-E08413m
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規格：96T/48T
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價格：￥3200/￥2500
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其他：
產品詳情
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產品描述：小鼠分泌型免疫球蛋白A(sIgA)酶聯免疫試劑盒（CSB-E08413m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、支氣管肺泡灌洗液樣本中的sIgA含量。sIgA即分泌型免疫球蛋白A，是黏膜免疫系統的主要效應分子。能夠在黏膜表面阻止病原體黏附與入侵，發揮抗感染等作用。研究機制主要圍繞其生成、分泌過程，以及與病原體相互作用等方面，以探索其在疾病防治中的應用潛力。試劑盒檢測范圍為15.6 ng/mL-1000 ng/mL，適用于科研場景中黏膜免疫相關動物模型的機制研究，例如呼吸道感染模型中局部免疫應答的動態監測、腸道炎癥疾病中黏膜屏障功能的評估，或藥物/益生菌干預后sIgA分泌水平的定量分析。其寬泛的樣本兼容性可支持多維度實驗設計，尤其適用于支氣管肺泡灌洗液等低濃度樣本的檢測需求，為黏膜免疫調控機制研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
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縮寫：sIgA
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種屬：Mus musculus (Mouse)
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樣本類型：serum, plasma, tissue homogenates, bronchoalveolar lavage fluid
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檢測范圍：15.6 ng/mL-1000 ng/mL
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靈敏度：3.9 ng/mL
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反應時間：1-5h
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樣本體積：50-100ul
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檢測波長：450 nm
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研究領域：Others
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測定原理：quantitative
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測定方法：Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度：
To assess the linearity of the assay, samples were spiked with high concentrations of mouse sIgA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:20 Average % 92 Range % 87-95 1:40 Average % 101 Range % 97-104 1:80 Average % 93 Range % 87-97 1:160 Average % 98 Range % 92-102 -
回收率：
The recovery of mouse sIgA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 94 90-97 EDTA plasma (n=4) 96 92-99 -
標準曲線：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
ng/ml OD1 OD2 Average Corrected 1000 2.486 2.466 2.476 2.312 500 1.772 1.841 1.807 1.643 250 1.232 1.222 1.227 1.063 125 0.760 0.748 0.754 0.590 62.5 0.464 0.481 0.473 0.309 31.2 0.325 0.312 0.319 0.155 15.6 0.239 0.229 0.234 0.070 0 0.166 0.162 0.164 -
數據處理：
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貨期：3-5 working days
引用文獻
- Investigating the effects of Laggera pterodonta on H3N2-Induced inflammatory and immune responses through network pharmacology, molecular docking, and experimental validation in a mice model Y Chen,Heliyon,2024
- Ficolin A knockout alleviates sepsis-induced severe lung injury in mice by restoring gut Akkermansia to inhibit S100A4/STAT3 pathway X Wu,International immunopharmacology,2023
- Involvement of gut-brain communication in arsenite-induced neurobehavioral impairments in adult male mice Z Lu,Ecotoxicology and Environmental Safety,2022
- Effects of draught on thermal comfort and respiratory immunity X Cheng,Building and Environment,2022
- Two Bordetella bronchiseptica attenuated vaccine candidates confer protection against lethal challenge with B. Bronchiseptica and Pasteurella multocida toxin in mouse models Y Zhang,Vaccine,2022
- Preventive effects of traditional Chinese medicine formula Huoxiangzhengqi against lipopolysaccharides-induced inflammatory response M Gao,Phytomedicine,2022
- Gut microbiota mediated the toxicity of high concentration of dietary nitrite in C57BL/6 mice Jing Xu,Ecotoxicology and environmental safety,2022
- Dietary Bacillus subtilis supplementation alleviates alcohol?induced liver injury by maintaining intestinal integrity and gut microbiota homeostasis in mice M Zhao,Experimental and Therapeutic Medicine,2021
- Pregnancy exposure of titanium dioxide nanoparticles causes intestinal dysbiosis and neurobehavioral impairments that are not significant postnatally but emerge in adulthood of offspring J Su,Journal of nanobiotechnology,2021
- Green tea polyphenol epigallocatechin-3-gallate alleviates nonalcoholic fatty liver disease and ameliorates intestinal immunity in mice fed a high-fat diet J Huang,Food & Function,2020
- Anti-fatigue effects of pea (Pisum sativum L.) peptides prepared by compound protease T Feng,Journal of Food Science and Technology,2020
- Arginine accelerates intestinal health through cytokines and intestinal microbiota Miaomiao Wu, et al,International Immunopharmacology,2019
- Dietary leucine supplementation improves intestinal health of mice through intestinal SIgA secretion Song B, et al,Journal of Applied Microbiology,2019
- Melatonin alleviates weanling stress in mice: involvement of intestinal microbiota Ren W.et al,J Pineal Res.,2017
- l-Glutamine and l-arginine protect against enterotoxigenic Escherichia coli infection via intestinal innate immunity in mice Liu G.et al,Amino Acids,2017
- Inhibition of RORrt activity and Th17 differentiation by a set of novel compounds Ding Q. et al,BMC Immunol.,2015
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