Mouse prothrombin fragment 1+2,F1+2 ELISA Kit

產(chǎn)品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

小鼠凝血酶原片段F1+2(F1+2)酶聯(lián)免疫試劑盒（CSB-E13265m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的F1+2含量。小鼠凝血酶原片段F1+2是凝血酶原激活轉化為凝血酶過程中釋放的穩(wěn)定降解產(chǎn)物，其含量反映凝血酶早期激活水平，可用于診斷血栓前狀態(tài)和血栓性疾病，是血栓性疾病早期診斷的分子標志物。試劑盒檢測范圍為15.6 pmol/L-1000 pmol/L，常用于評估凝血系統(tǒng)活化狀態(tài)，在血栓形成機制研究、抗凝藥物效果評價及凝血相關疾病動物模型（如心血管疾病模型或遺傳性凝血障礙研究）中具有重要科研價值。該產(chǎn)品適用于體外實驗，能夠靈敏檢測多種生物樣本中F1+2的動態(tài)變化，為凝血與纖溶平衡研究、病理模型建立及藥物開發(fā)提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

F1+2
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

15.6 pmol/L-1000 pmol/L
靈敏度：

3.9 pmol/L
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Others
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse F1+2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	85
	Range %	81-89
1:2	Average %	109
	Range %	104-112
1:4	Average %	98
	Range %	94-102
1:8	Average %	92
	Range %	87-98

回收率：

The recovery of mouse F1+2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	94-99
EDTA plasma (n=4)	91	87-96

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.