Mouse procollagen type III (HPCⅢ) ELISA Kit

文獻引用 (3) 說明書下載

中文名稱：

小鼠Ⅲ型前膠原（HPCⅢ）酶聯免疫試劑盒
貨號：

CSB-E17125m
規格：

96T/48T
價格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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小鼠Ⅲ型前膠原（HPCⅢ）酶聯免疫試劑盒（CSB-E17125m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養上清液、組織勻漿樣本中的HPCⅢ含量。小鼠Ⅲ型前膠原（HPCⅢ）是細胞外基質（ECM）的重要組成部分，其合成與降解失衡是肝纖維化的關鍵因素。砷暴露可誘導HPCⅢ表達增加，促進肝星狀細胞（HSC）活化，導致ECM過度沉積，進而引發肝纖維化。研究揭示，HPCⅢ在肝纖維化發生發展中扮演著重要角色。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL，適用于基礎科研中纖維化疾病機制或組織修復相關研究。該產品廣泛應用于肝纖維化模型、肺纖維化模型或皮膚損傷修復等動物實驗研究，為探索HPCⅢ在疾病發生發展中的作用或評價抗纖維化藥物效果提供可靠工具，適用于體外實驗環境下的濃度測定及動態變化分析。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

procollagen type 3 ,HPC3|procollagen type III ,HPCⅢ procollagen type III ,HPCIII
縮寫：

HPCⅢ
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates
檢測范圍：

15.6 pg/mL-1000 pg/mL
靈敏度：

3.9 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse HPCⅢ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	97
	Range %	92-101
1:200	Average %	95
	Range %	90-100
1:400	Average %	91
	Range %	85-95
1:800	Average %	102
	Range %	98-107

回收率：

The recovery of mouse HPCⅢ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	85-93
EDTA plasma (n=4)	84	80-88

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	2.492	2.343	2.418	2.316
500	1.825	1.716	1.771	1.669
250	0.947	1.004	0.976	0.874
125	0.571	0.582	0.577	0.475
62.5	0.359	0.339	0.349	0.247
31.2	0.246	0.237	0.242	0.140
15.6	0.164	0.171	0.168	0.066
0	0.098	0.105	0.102

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

引用文獻

Farnesoid X receptor activation is required for the anti-inflammatory and anti-oxidative stress effects of Alisol B 23-acetate in carbon tetrachloride-induced liver fibrosis in mice L Zhang,International immunopharmacology,2023
Oxyberberine suppressed the carbon tetrachloride-induced liver fibrosis by inhibiting liver inflammation in a sirtuin 3-dependent manner in mice S Zhao,International Immunopharmacology,2023
SIRT3 Facilitates Amniotic Fluid Stem Cells to Repair Diabetic Nephropathy Through Protecting Mitochondrial Homeostasis by Modulation of Mitophagy Feng J.et al,Cellular Physiology and Biochemistry,2018

Mouse procollagen type III (HPCⅢ) ELISA Kit

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