Mouse platelet activating factor,PAF ELISA KIT

產(chǎn)品詳情

文獻(xiàn)引用 (5) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

小鼠血小板活化因子(PAF)酶聯(lián)免疫試劑盒（CSB-E08199m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的PAF含量。PAF即血小板活化因子，是一種強(qiáng)效生物活性磷脂。它在炎癥、過敏等多種病理生理過程中發(fā)揮關(guān)鍵作用。研究機(jī)制主要圍繞其與細(xì)胞表面受體結(jié)合，激活細(xì)胞內(nèi)信號傳導(dǎo)通路，引發(fā)一系列生物學(xué)效應(yīng)，對其研究有助于開發(fā)相關(guān)治療藥物。試劑盒檢測范圍為0.78 ng/mL-50 ng/mL，廣泛應(yīng)用于心血管疾病、免疫調(diào)節(jié)、炎癥模型構(gòu)建等基礎(chǔ)研究領(lǐng)域，尤其適用于體外實驗中PAF動態(tài)變化的定量監(jiān)測，為探索相關(guān)信號通路及藥物作用靶點提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

N/A
縮寫：

PAF
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates
檢測范圍：

0.78 ng/mL-50 ng/mL
靈敏度：

0.195 ng/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Biochemicals
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse PAF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	86-98
1:2	Average %	97
	Range %	92-107
1:4	Average %	93
	Range %	87-98
1:8	Average %	87
	Range %	82-94

回收率：

The recovery of mouse PAF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-97
EDTA plasma (n=4)	97	95-101

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.